TY - GEN
T1 - Predicting melanoma metastatic potential by optical and magnetic resonance imaging
AU - Li, Lin Z.J.
AU - Zhou, Rong
AU - Zhong, Tuoxiu
AU - Moon, Lily
AU - Kim, Eun Ju
AU - Hui, Qiao
AU - Pickup, Stephen
AU - Hendrix, Mary J.
AU - Leeper, Dennis
AU - Chance, Britton
AU - Glickson, Jerry D.
PY - 2008
Y1 - 2008
N2 - Accurate prediction of tumor metastatic potential would be helpful in treatment planning and in the design of agents that modify the tumor phenotype. We report that three methods that are potentially transferable to the clinic - dynamic contrast enhanced MRI (DCE MRI), T1ρ-weighted imaging and low temperature fluorescence imaging (that could be performed on biopsy specimens) - distinguished between relatively indolent (A375P) and aggressive (C8161) metastatic human melanoma xenografts in nude mice, whereas T1 and T2 relaxation time measurements did not. DCE MRI data analyzed by the BOLus Enhanced Relaxation Overview (BOLERO) method in conjunction with concurrent measurements of the arterial input function yielded a blood transfer rate constant (Ktrans) which measures perfusion/permeability, that was significantly higher in the core of the indolent tumor than in the core of the aggressive tumor. Histological staining indicated that aggressive tumors had more blood vascular structure but fewer functional vascular structure than indolent tumors. Indolent tumors exhibited T1ρ values that were significantly higher than those of aggressive tumors at spin-locking frequencies >500Hz. The mitochondrial redox ratio, Fp/(Fp+NADH), where Fp and NADH are the fluorescence of oxidized flavoproteins and reduced pyridine nucleotides, respectively, of aggressive tumors was much higher (more oxidized) than that of indolent tumors and often showed a bimodal distribution with an oxidized core and a reduced rim. These differences observed between these two types of tumors, one indolent and one aggressive, if generalizable, would be very valuable in predicting human melanoma metastatic potential.
AB - Accurate prediction of tumor metastatic potential would be helpful in treatment planning and in the design of agents that modify the tumor phenotype. We report that three methods that are potentially transferable to the clinic - dynamic contrast enhanced MRI (DCE MRI), T1ρ-weighted imaging and low temperature fluorescence imaging (that could be performed on biopsy specimens) - distinguished between relatively indolent (A375P) and aggressive (C8161) metastatic human melanoma xenografts in nude mice, whereas T1 and T2 relaxation time measurements did not. DCE MRI data analyzed by the BOLus Enhanced Relaxation Overview (BOLERO) method in conjunction with concurrent measurements of the arterial input function yielded a blood transfer rate constant (Ktrans) which measures perfusion/permeability, that was significantly higher in the core of the indolent tumor than in the core of the aggressive tumor. Histological staining indicated that aggressive tumors had more blood vascular structure but fewer functional vascular structure than indolent tumors. Indolent tumors exhibited T1ρ values that were significantly higher than those of aggressive tumors at spin-locking frequencies >500Hz. The mitochondrial redox ratio, Fp/(Fp+NADH), where Fp and NADH are the fluorescence of oxidized flavoproteins and reduced pyridine nucleotides, respectively, of aggressive tumors was much higher (more oxidized) than that of indolent tumors and often showed a bimodal distribution with an oxidized core and a reduced rim. These differences observed between these two types of tumors, one indolent and one aggressive, if generalizable, would be very valuable in predicting human melanoma metastatic potential.
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U2 - 10.1007/978-0-387-71764-7_10
DO - 10.1007/978-0-387-71764-7_10
M3 - Conference contribution
C2 - 17727249
AN - SCOPUS:84934438863
SN - 9780387717630
T3 - Advances in Experimental Medicine and Biology
SP - 67
EP - 78
BT - Oxygen Transport to Tissue XXVIII
PB - Springer New York
ER -