Preferential dendritic localization of pericentriolar material in hippocampal pyramidal neurons in culture

Adriana Ferreira*, Robert E. Palazzo, Lionel I. Rebhun

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Centrosomes are unique cytoplasmic structures which serve as microtubule organizing centers (MTOC). In most animal cells centrosomes consist of one or more pair of centrioles surrounded by electron dense amorphous pericentriolar material (PCM) responsible for nucleation of microtubules. In the present study we analyzed the pattern of induction and localization of proteins of the PCM at different stages of neuronal development in cell cultures prepared from the embryonic hippocampus. For this purpose we used a human polyclonal antibody that recognizes two proteins of the PCM (100 kd and 60 kd, respectively). The results indicate that in mature neurons, pericentriolar immunoreactive material is preferentially localized in dendritic processes, and that throughout the course of neurite development and differentiation it is systematically excluded from the neuron's axon. Western blot analysis showed that during neuronal development in situ, there is an increase in he immunoreactivity for both proteins recognized by this antibody. In contrast, in hippocampal pyramidal neurons that develop in culture, there is an increase in the 60 kd polypeptide, while the 100 kd one is not detected after 7 days in vitro. © 1993 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)336-344
Number of pages9
JournalCell Motility and the Cytoskeleton
Volume25
Issue number4
DOIs
StatePublished - Jan 1 1993

Keywords

  • MTOC
  • dendrites
  • neurite extension

ASJC Scopus subject areas

  • Structural Biology
  • Cell Biology

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