Preferentially quantized linker DNA lengths in Saccharomyces cerevisiae

Jiping Wang*, Yvonne Fondufe-Mittendorf, Liqun Xi, Guei Feng Tsai, Eran Segal, Jonathan Widom

*Corresponding author for this work

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

The exact lengths of linker DNAs connecting adjacent nucleosomes specify the intrinsic three-dimensional structures of eukaryotic chromatin fibers. Some studies suggest that linker DNA lengths preferentially occur at certain quantized values, differing one from another by integral multiples of the DNA helical repeat, ∼10 bp; however, studies in the literature are inconsistent. Here, we investigate linker DNA length distributions in the yeast Saccharomyces cerevisiae genome, using two novel methods: a Fourier analysis of genomic dinucleotide periodicities adjacent to experimentally mapped nucleosomes and a duration hidden Markov model applied to experimentally defined dinucleosomes. Both methods reveal that linker DNA lengths in yeast are preferentially periodic at the DNA helical repeat (∼10 bp), obeying the forms 10n+5 bp (integer n). This 10 bp periodicity implies an ordered superhelical intrinsic structure for the average chromatin fiber in yeast.

Original languageEnglish (US)
Article numbere1000175
JournalPLoS Computational Biology
Volume4
Issue number9
DOIs
StatePublished - Sep 1 2008

    Fingerprint

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Ecology
  • Molecular Biology
  • Genetics
  • Ecology, Evolution, Behavior and Systematics
  • Modeling and Simulation
  • Computational Theory and Mathematics

Cite this

Wang, J., Fondufe-Mittendorf, Y., Xi, L., Tsai, G. F., Segal, E., & Widom, J. (2008). Preferentially quantized linker DNA lengths in Saccharomyces cerevisiae. PLoS Computational Biology, 4(9), [e1000175]. https://doi.org/10.1371/journal.pcbi.1000175