Preimplantation and postimplantation development of rat embryos cloned with cumulus cells and fibroblasts

Philip Iannaccone*, Greg Taborn, Ray Garton

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

In this report we demonstrate the successful in vitro culture of fertilised embryos from 1-cell to blastocyst stage, albeit in a strain-dependent fashion. We report procedures for the enucleation of rat oocytes; nuclear transfer by injection of nuclei (NT) from adult rat cumulus cells, rat primary embryonic fibroblasts and genetically modified rat fibroblasts; and activation resulting in advanced preimplantation development. Blastocyst stage rat embryos were obtained after in vitro culture of nuclear transfer zygotes at similar frequencies with each of these nuclear donor cell types. Transfer of NT embryos to surrogate mothers leads to implantation of 24% of the zygotes. These results suggest that the nuclei of cultured rat cells, even following genetic modification, can be reprogrammed to support early embryonic development, which is a prerequisite to cloning the rat.

Original languageEnglish (US)
Pages (from-to)135-143
Number of pages9
JournalZygote
Volume9
Issue number2
DOIs
StatePublished - May 2001

Keywords

  • Cloning
  • Embryo culture
  • Nuclear transfer
  • Rat
  • Zygotic development

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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