Prenatal nicotine exposure alters respiratory long-term facilitation in neonatal rats

D. D. Fuller; B. J. Dougherty; M. S. Sandhu; N. J. Doperalski; C. R. Reynolds; L. F. Hayward

doi:10.1016/j.resp.2009.09.015

Prenatal nicotine exposure alters respiratory long-term facilitation in neonatal rats

D. D. Fuller^*, B. J. Dougherty, M. S. Sandhu, N. J. Doperalski, C. R. Reynolds, L. F. Hayward

^*Corresponding author for this work

Physical Medicine and Rehabilitation

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Intermittent hypoxia can evoke persistent increases in ventilation (over(V, ̇)_E) in neonates (i.e. long-term facilitation, LTF) (Julien et al., 2008). Since prenatal nicotine (PN) exposure alters neonatal respiratory control (Fregosi and Pilarski, 2008), we hypothesized that PN would influence LTF of ventilation (over(V, ̇)_E) in neonatal rats. An osmotic minipump delivered nicotine 6 mg/kg per day or saline to pregnant dams. over(V, ̇)_E was assessed in unanesthetized pups via whole body plethysmography at post-natal (P) days 9-11 or 15-17 during baseline (BL, 21% O₂), hypoxia (10 × 5 min, 5% O₂) and 30 min post-hypoxia. PN pups had reduced BL over(V, ̇)_E (p < 0.05) but greater increases in over(V, ̇)_E during hypoxia (p < 0.05). Post-hypoxia over(V, ̇)_E (i.e. LTF) showed an age × treatment interaction (p < 0.01) with similar values at P9-11 but enhanced LTF in saline (30 ± 8%BL) vs. PN pups (6 ± 5%BL; p = 0.01) at P15-17. We conclude that the post-natal developmental time course of hypoxia-induced LTF is influenced by PN.

Original language	English (US)
Pages (from-to)	333-337
Number of pages	5
Journal	Respiratory Physiology and Neurobiology
Volume	169
Issue number	3
DOIs	https://doi.org/10.1016/j.resp.2009.09.015
State	Published - Dec 31 2009

Funding

Support for this work was provided by a grant from the James & Esther King Biomedical Research Program. Support was also provided by NIH grant T32HD043730T32 (B.J.D.). The authors thank Dr. Ralph F. Fregosi (University of Arizona) for help with the osmotic pumps and advice regarding the data. We also thank Ms. Heather Carr for help with data collection and analyses.

Keywords

Developmental plasticity
Hypoxia
Neonatal
Nicotine
Prenatal
Ventilation

ASJC Scopus subject areas

Physiology
General Neuroscience
Pulmonary and Respiratory Medicine

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10.1016/j.resp.2009.09.015

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title = "Prenatal nicotine exposure alters respiratory long-term facilitation in neonatal rats",

abstract = "Intermittent hypoxia can evoke persistent increases in ventilation (over(V,{\. })E) in neonates (i.e. long-term facilitation, LTF) (Julien et al., 2008). Since prenatal nicotine (PN) exposure alters neonatal respiratory control (Fregosi and Pilarski, 2008), we hypothesized that PN would influence LTF of ventilation (over(V,{\. })E) in neonatal rats. An osmotic minipump delivered nicotine 6 mg/kg per day or saline to pregnant dams. over(V,{\. })E was assessed in unanesthetized pups via whole body plethysmography at post-natal (P) days 9-11 or 15-17 during baseline (BL, 21% O2), hypoxia (10 × 5 min, 5% O2) and 30 min post-hypoxia. PN pups had reduced BL over(V,{\. })E (p < 0.05) but greater increases in over(V,{\. })E during hypoxia (p < 0.05). Post-hypoxia over(V,{\. })E (i.e. LTF) showed an age × treatment interaction (p < 0.01) with similar values at P9-11 but enhanced LTF in saline (30 ± 8%BL) vs. PN pups (6 ± 5%BL; p = 0.01) at P15-17. We conclude that the post-natal developmental time course of hypoxia-induced LTF is influenced by PN.",

keywords = "Developmental plasticity, Hypoxia, Neonatal, Nicotine, Prenatal, Ventilation",

author = "Fuller, {D. D.} and Dougherty, {B. J.} and Sandhu, {M. S.} and Doperalski, {N. J.} and Reynolds, {C. R.} and Hayward, {L. F.}",

note = "Funding Information: Support for this work was provided by a grant from the James & Esther King Biomedical Research Program. Support was also provided by NIH grant T32HD043730T32 (B.J.D.). The authors thank Dr. Ralph F. Fregosi (University of Arizona) for help with the osmotic pumps and advice regarding the data. We also thank Ms. Heather Carr for help with data collection and analyses.",

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language = "English (US)",

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T1 - Prenatal nicotine exposure alters respiratory long-term facilitation in neonatal rats

AU - Fuller, D. D.

AU - Dougherty, B. J.

AU - Sandhu, M. S.

AU - Doperalski, N. J.

AU - Reynolds, C. R.

AU - Hayward, L. F.

N1 - Funding Information: Support for this work was provided by a grant from the James & Esther King Biomedical Research Program. Support was also provided by NIH grant T32HD043730T32 (B.J.D.). The authors thank Dr. Ralph F. Fregosi (University of Arizona) for help with the osmotic pumps and advice regarding the data. We also thank Ms. Heather Carr for help with data collection and analyses.

PY - 2009/12/31

Y1 - 2009/12/31

N2 - Intermittent hypoxia can evoke persistent increases in ventilation (over(V, ̇)E) in neonates (i.e. long-term facilitation, LTF) (Julien et al., 2008). Since prenatal nicotine (PN) exposure alters neonatal respiratory control (Fregosi and Pilarski, 2008), we hypothesized that PN would influence LTF of ventilation (over(V, ̇)E) in neonatal rats. An osmotic minipump delivered nicotine 6 mg/kg per day or saline to pregnant dams. over(V, ̇)E was assessed in unanesthetized pups via whole body plethysmography at post-natal (P) days 9-11 or 15-17 during baseline (BL, 21% O2), hypoxia (10 × 5 min, 5% O2) and 30 min post-hypoxia. PN pups had reduced BL over(V, ̇)E (p < 0.05) but greater increases in over(V, ̇)E during hypoxia (p < 0.05). Post-hypoxia over(V, ̇)E (i.e. LTF) showed an age × treatment interaction (p < 0.01) with similar values at P9-11 but enhanced LTF in saline (30 ± 8%BL) vs. PN pups (6 ± 5%BL; p = 0.01) at P15-17. We conclude that the post-natal developmental time course of hypoxia-induced LTF is influenced by PN.

AB - Intermittent hypoxia can evoke persistent increases in ventilation (over(V, ̇)E) in neonates (i.e. long-term facilitation, LTF) (Julien et al., 2008). Since prenatal nicotine (PN) exposure alters neonatal respiratory control (Fregosi and Pilarski, 2008), we hypothesized that PN would influence LTF of ventilation (over(V, ̇)E) in neonatal rats. An osmotic minipump delivered nicotine 6 mg/kg per day or saline to pregnant dams. over(V, ̇)E was assessed in unanesthetized pups via whole body plethysmography at post-natal (P) days 9-11 or 15-17 during baseline (BL, 21% O2), hypoxia (10 × 5 min, 5% O2) and 30 min post-hypoxia. PN pups had reduced BL over(V, ̇)E (p < 0.05) but greater increases in over(V, ̇)E during hypoxia (p < 0.05). Post-hypoxia over(V, ̇)E (i.e. LTF) showed an age × treatment interaction (p < 0.01) with similar values at P9-11 but enhanced LTF in saline (30 ± 8%BL) vs. PN pups (6 ± 5%BL; p = 0.01) at P15-17. We conclude that the post-natal developmental time course of hypoxia-induced LTF is influenced by PN.

KW - Developmental plasticity

KW - Hypoxia

KW - Neonatal

KW - Nicotine

KW - Prenatal

KW - Ventilation

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ER -

Prenatal nicotine exposure alters respiratory long-term facilitation in neonatal rats

Abstract

Funding

Keywords

ASJC Scopus subject areas

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