Preparation and use of wireless reprogrammable multilateral optogenetic devices for behavioral neuroscience

Yiyuan Yang, Mingzheng Wu, Amy J. Wegener, Abraham Vázquez-Guardado, Andrew I. Efimov, Ferrona Lie, Taoyi Wang, Yuhang Ma, Anthony Banks, Zhengwei Li, Zhaoqian Xie, Yonggang Huang, Cameron H. Good, Yevgenia Kozorovitskiy, John A. Rogers*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

7 Scopus citations

Abstract

Wireless battery-free optogenetic devices enable behavioral neuroscience studies in groups of animals with minimal interference to natural behavior. Real-time independent control of optogenetic stimulation through near-field communication dramatically expands the realm of applications of these devices in broad contexts of neuroscience research. Dissemination of these tools with advanced functionalities to the neuroscience community requires protocols for device manufacturing and experimental implementation. This protocol describes detailed procedures for fabrication, encapsulation and implantation of recently developed advanced wireless devices in head- and back-mounted forms. In addition, procedures for standard implementation of experimental systems in mice are provided. This protocol aims to facilitate the application of wireless optogenetic devices in advanced optogenetic experiments involving groups of freely moving rodents and complex environmental designs. The entire protocol lasts ~3–5 weeks.

Original languageEnglish (US)
Pages (from-to)1073-1096
Number of pages24
JournalNature Protocols
Volume17
Issue number4
DOIs
StatePublished - Apr 2022

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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