Preparation of bioconjugates of CdTe nanocrystals for cancer marker detection

Fengqin Hu, Yuliang Ran*, Zhuan Zhou, Mingyuan Gao

*Corresponding author for this work

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

Highly fluorescent CdTe quantum dots (Q-dots) stabilized by 3-mercaptopropionic acid (MPA) were prepared by an aqueous solution approach and used as fluorescent labels in detecting a cancer marker, carcinoembryonic antigen (CEA), expressed on human colon carcinoma cell line LS 180. Nonspecific adsorptions of CdTe Q-dots on carcinoma cells were observed and effectively eliminated by replacing MPA with a thiolated PEG (poly(ethylene glycol), Mn ≤ 750) synthesized according to literature. It was unexpectedly found out that the PEG-coated CdTe Q-dots exhibited very strong and specific affinity to anti-CEA monoclonal antibody rch 24 (rch 24mAb). The resultant CdTe-(rch 24mAb) conjugates were successfully used in detections of CEA expressed on the surface of cell line LS 180. Further experiments demonstrated that the fluorescent CdTe Q-dots exhibited much better photostability and a brighter fluorescence than FITC, which consequently led to a higher efficiency in the cancer marker detection.

Original languageEnglish (US)
Article number025
Pages (from-to)2972-2977
Number of pages6
JournalNanotechnology
Volume17
Issue number12
DOIs
StatePublished - Jun 28 2006

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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