Presence of both mesenchymal and carcinomatous features in an In-Vitro model of ovarian carcinosarcoma derived from patients’ ascitic fluid

Ahmad Shariftabrizi, Ilenia Pellicciotta, Amer Abdullah*, Charis Anne Venditti, Robert Samuelson, Shohreh Shahabi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

We have refined the technique for isolating and propagating cultures of primary ovarian carcinosarcoma cells (OSCs) derived from ascites, which allowed the cells to obtain the biphasic features of carcinosarcoma in cell culture conditions (presence of both carcinoma and mesenchymal morphologic types). This protocol involves a simple yet rapid method for the growth and propagation of ascites OSC in a basal culture medium. Autologous ascitic fluid was used as source of growth factors, and minimal manipulation was involved to establish the culture. The methodology allowed for the direct application of multiple molecular, cellular, and functional analyses within a few weeks of initial cell isolation, with the further potential of retrospective analyses of archived cells and tissues.

Original languageEnglish (US)
Pages (from-to)1-4
Number of pages4
JournalInternational Journal of Hematology-Oncology and Stem Cell Research
Volume9
Issue number1
StatePublished - Jan 1 2015

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Transplantation

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