Presynaptic inhibition of GABAergic inputs to rat substantia nigra pars reticulata neurones by a cannabinoid agonist

Priscilla K Y Chan, Savio C Y Chan, Wing Ho Yung*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

Whole-cell patch-clamp recordings were made from substantia nigra pars reticulata (SNR) neurones in rat midbrain slices to investigate the electrophysiological effects of cannabinoids. The cannabinoid receptor agonist WIN 55212-2 =(10 μM) significantly reduced intranigrally evoked and spontaneous inhibitory postsynaptic currents (IPSCs) which were mediated by GABA(A) receptors. The postsynaptic current induced by bath application of GABA was not affected by the presence of WIN 55212-2. The actions of WIN 55212-2 were not mimicked by the inactive enantiomer WIN 55212-3. WIN 55212- 2 also hyperpolarized the membrane of SNR neurones in a tetrodotoxin/0- Ca2+-insensitive manner. These data suggest that cannabinoids modulate the activity of SNR neurones by presynaptic inhibition of GABA inputs. They may also exert a direct post-synaptic inhibition on these neurones.

Original languageEnglish (US)
Pages (from-to)671-675
Number of pages5
JournalNeuroReport
Volume9
Issue number4
DOIs
StatePublished - Mar 9 1998

Keywords

  • Cannabinoid
  • GABA
  • IPSC
  • Substantia nigra

ASJC Scopus subject areas

  • Neuroscience(all)

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