Presynaptic inhibition of GABAergic inputs to rat substantia nigra pars reticulata neurones by a cannabinoid agonist

Priscilla K Y Chan; Savio C Y Chan; Wing Ho Yung

doi:10.1097/00001756-199803090-00020

Presynaptic inhibition of GABAergic inputs to rat substantia nigra pars reticulata neurones by a cannabinoid agonist

Priscilla K Y Chan, Savio C Y Chan, Wing Ho Yung^*

^*Corresponding author for this work

Neuroscience

Research output: Contribution to journal › Article › peer-review

81 Scopus citations

Abstract

Whole-cell patch-clamp recordings were made from substantia nigra pars reticulata (SNR) neurones in rat midbrain slices to investigate the electrophysiological effects of cannabinoids. The cannabinoid receptor agonist WIN 55212-2 =(10 μM) significantly reduced intranigrally evoked and spontaneous inhibitory postsynaptic currents (IPSCs) which were mediated by GABA(A) receptors. The postsynaptic current induced by bath application of GABA was not affected by the presence of WIN 55212-2. The actions of WIN 55212-2 were not mimicked by the inactive enantiomer WIN 55212-3. WIN 55212- 2 also hyperpolarized the membrane of SNR neurones in a tetrodotoxin/0- Ca²⁺-insensitive manner. These data suggest that cannabinoids modulate the activity of SNR neurones by presynaptic inhibition of GABA inputs. They may also exert a direct post-synaptic inhibition on these neurones.

Original language	English (US)
Pages (from-to)	671-675
Number of pages	5
Journal	Neuroreport
Volume	9
Issue number	4
DOIs	https://doi.org/10.1097/00001756-199803090-00020
State	Published - Mar 9 1998

Keywords

Cannabinoid
GABA
IPSC
Substantia nigra

ASJC Scopus subject areas

General Neuroscience

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10.1097/00001756-199803090-00020

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title = "Presynaptic inhibition of GABAergic inputs to rat substantia nigra pars reticulata neurones by a cannabinoid agonist",

abstract = "Whole-cell patch-clamp recordings were made from substantia nigra pars reticulata (SNR) neurones in rat midbrain slices to investigate the electrophysiological effects of cannabinoids. The cannabinoid receptor agonist WIN 55212-2 =(10 μM) significantly reduced intranigrally evoked and spontaneous inhibitory postsynaptic currents (IPSCs) which were mediated by GABA(A) receptors. The postsynaptic current induced by bath application of GABA was not affected by the presence of WIN 55212-2. The actions of WIN 55212-2 were not mimicked by the inactive enantiomer WIN 55212-3. WIN 55212- 2 also hyperpolarized the membrane of SNR neurones in a tetrodotoxin/0- Ca2+-insensitive manner. These data suggest that cannabinoids modulate the activity of SNR neurones by presynaptic inhibition of GABA inputs. They may also exert a direct post-synaptic inhibition on these neurones.",

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AU - Chan, Priscilla K Y

AU - Chan, Savio C Y

AU - Yung, Wing Ho

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Y1 - 1998/3/9

N2 - Whole-cell patch-clamp recordings were made from substantia nigra pars reticulata (SNR) neurones in rat midbrain slices to investigate the electrophysiological effects of cannabinoids. The cannabinoid receptor agonist WIN 55212-2 =(10 μM) significantly reduced intranigrally evoked and spontaneous inhibitory postsynaptic currents (IPSCs) which were mediated by GABA(A) receptors. The postsynaptic current induced by bath application of GABA was not affected by the presence of WIN 55212-2. The actions of WIN 55212-2 were not mimicked by the inactive enantiomer WIN 55212-3. WIN 55212- 2 also hyperpolarized the membrane of SNR neurones in a tetrodotoxin/0- Ca2+-insensitive manner. These data suggest that cannabinoids modulate the activity of SNR neurones by presynaptic inhibition of GABA inputs. They may also exert a direct post-synaptic inhibition on these neurones.

AB - Whole-cell patch-clamp recordings were made from substantia nigra pars reticulata (SNR) neurones in rat midbrain slices to investigate the electrophysiological effects of cannabinoids. The cannabinoid receptor agonist WIN 55212-2 =(10 μM) significantly reduced intranigrally evoked and spontaneous inhibitory postsynaptic currents (IPSCs) which were mediated by GABA(A) receptors. The postsynaptic current induced by bath application of GABA was not affected by the presence of WIN 55212-2. The actions of WIN 55212-2 were not mimicked by the inactive enantiomer WIN 55212-3. WIN 55212- 2 also hyperpolarized the membrane of SNR neurones in a tetrodotoxin/0- Ca2+-insensitive manner. These data suggest that cannabinoids modulate the activity of SNR neurones by presynaptic inhibition of GABA inputs. They may also exert a direct post-synaptic inhibition on these neurones.

KW - Cannabinoid

KW - GABA

KW - IPSC

KW - Substantia nigra

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Presynaptic inhibition of GABAergic inputs to rat substantia nigra pars reticulata neurones by a cannabinoid agonist

Abstract

Keywords

ASJC Scopus subject areas

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