@article{af8abc52351343cd99b19830fd3737fe,
title = "Prevention of vascular-allograft rejection by protecting the endothelial glycocalyx with immunosuppressive polymers",
abstract = "Systemic immunosuppression for the mitigation of immune rejection after organ transplantation causes adverse side effects and constrains the long-term benefits of the transplanted graft. Here we show that protecting the endothelial glycocalyx in vascular allografts via the enzymatic ligation of immunosuppressive glycopolymers under cold-storage conditions attenuates the acute and chronic rejection of the grafts after transplantation in the absence of systemic immunosuppression. In syngeneic and allogeneic mice that received kidney transplants, the steric and immunosuppressive properties of the ligated polymers largely protected the transplanted grafts from ischaemic reperfusion injury, and from immune-cell adhesion and thereby immunocytotoxicity. Polymer-mediated shielding of the endothelial glycocalyx following organ procurement should be compatible with clinical procedures for transplant preservation and perfusion, and may reduce the damage and rejection of transplanted organs after surgery.",
author = "Siren, {Erika M.J.} and Luo, {Haiming D.} and Franklin Tam and Ashani Montgomery and Winnie Enns and Haisle Moon and Lyann Sim and Kevin Rey and Qiunong Guan and Wang, {Jiao Jing} and Wardell, {Christine M.} and Mahdis Monajemi and Majid Mojibian and Levings, {Megan K.} and Zhang, {Zheng J.} and Caigan Du and Withers, {Stephen G.} and Choy, {Jonathan C.} and Kizhakkedathu, {Jayachandran N.}",
note = "Funding Information: We thank the Macromolecular Hub, CBR, for the use of their research facilities, and I. R. Nabi for access to the UBC imaging facility. We acknowledge funding from the Canadian Institutes of Health Research (CIHR; to J.N.K., J.C.C., CIHR FDN-154304 to M.K.L. and CIHR foundation grant to S.G.W.), the Natural Sciences and Engineering Council of Canada (NSERC; to J.N.K.), the Heart and Stroke Foundation of Canada (to J.C.C.) and the Canadian Glycomics Network of Centres of Excellence, GlycoNet. The infrastructure facility is supported by the Canada Foundation for Innovation (CFI) and the British Columbia Knowledge Development Fund (BCKDF). J.N.K. holds a Career Investigator Scholar award from the Michael Smith Foundation for Health Research (MSFHR). E.M.J.S. acknowledges support from a Centre for Blood Research graduate award. H.D.L. acknowledges funding from NSERC CGS-M, the NSERC CREATE NanoMat Program and NSERC PGS-D. H.M. acknowledges funding from the NSERC CREATE NanoMat Program and the Canadian Blood Services. M.K.L. receives a salary award from the BC Children{\textquoteright}s Hospital Research Institute. Publisher Copyright: {\textcopyright} 2021, The Author(s), under exclusive licence to Springer Nature Limited.",
year = "2021",
month = oct,
doi = "10.1038/s41551-021-00777-y",
language = "English (US)",
volume = "5",
pages = "1202--1216",
journal = "Nature Biomedical Engineering",
issn = "2157-846X",
publisher = "Nature Publishing Group",
number = "10",
}