Primed status of transitional B cells associated with their presence in the cerebrospinal fluid in early phases of multiple sclerosis

Catalina Lee Chang, Isabelle Top, Hélène Zéphir, Sylvain Dubucquoi, Jacques Trauet, Patricia Dussart, Lionel Prin, Patrick Vermersch*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

In the present study we showed that transitional B cells of patients with clinically isolated syndrome (CIS) and relapsing-remitting multiple sclerosis (RR-MS) are reduced in the peripheral blood (PB) (5.5- and 3.7-fold, respectively). In addition, these cells appeared to up-regulate different integrins (α4 and β1). These observations were associated with a primed cellular status, confirmed by an increased proportion of circulating CD80+ transitional B cells. Interestingly, these results correlate with presence of transitional B cells in the CSF. Furthermore, these cells were absent in the CSF of individuals with other inflammatory neurological disease, and their levels in paired PB and CD80 expression were normal. Altogether, our data revealed that a differential primed status of transitional B cells is a characteristic feature of early phases of MS disease, and this functional status is associated with the ability of these cells to cross the blood-CSF barrier.

Original languageEnglish (US)
Pages (from-to)12-20
Number of pages9
JournalClinical Immunology
Volume139
Issue number1
DOIs
StatePublished - Apr 2011

Funding

We greatly acknowledge Olivier Outteryck, MD, and Anne-Sophie Berteloot, MD, as well as all interns and nurses (Neurologie D Service, Pôle de Neurologie, CHRU of Lille) for assistance with patient recruitment and managing the hard task of sample supply. We also thank Miriam Labalette, MD, PhD, and her collaborators (Pôle d'Immunologie EA2686, CHRU of Lille) for her technical support concerning the flow cytometry platform, and Bénédicte Oxombre-Vanteghem, PhD, for critical review of this manuscript. This work was supported by the Conseil Régional Nord-Pas-de-Calais and Centre Hospitalier Régionale et Universitaire (CHRU) of Lille , the Bureau Biologie Santé (Conseil Régionale Nord-Pas-de-Calais) , and Bayer-Schering-Pharma . The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Keywords

  • Cerebrospinal fluid
  • Clinically isolated syndrome
  • Integrins
  • Multiple sclerosis
  • Transitional B-cell

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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