Primers for 22 candidate genes for ecological adaptations in Brassicaceae

H. Kuittinen*, M. Aguadé, D. Charlesworth, A. D.E. Haan, B. Lauga, T. Mitchell-Olds, S. Oikarinen, S. Ramos-Onsins, B. Stranger, P. Van Tienderen, O. Savolainen

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

29 Scopus citations

Abstract

There is an increasing interest in direct screening of polymorphisms at candidate loci to associate them with adaptations in natural situations. We report primers that amplify regions at 22 putatively orthologous functional loci in the family Brassicaceae: Arabidopsis thaliana, its two wild outcrossing relatives, and Brassica oleracea. Four groups of genes were known to have specific functions in the model species A. thaliana in ecologically important traits: timing of reproduction; metabolism of secondary compounds; defence against pathogens or light perception. In addition, genes of a fifth group were selected with no consideration of their function, as reference loci.

Original languageEnglish (US)
Pages (from-to)258-262
Number of pages5
JournalMolecular Ecology Notes
Volume2
Issue number3
DOIs
StatePublished - Sep 2002

Keywords

  • Arabidopsis
  • Candidate genes
  • Defence-genes
  • Flowering time
  • Molecular adaptation
  • Phenylpropanoid pathway
  • Phytochromes

ASJC Scopus subject areas

  • Biochemistry
  • Ecology
  • General Biochemistry, Genetics and Molecular Biology

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