Primers for 22 candidate genes for ecological adaptations in Brassicaceae

H. Kuittinen; M. Aguadé; D. Charlesworth; A. D.E. Haan; B. Lauga; T. Mitchell-Olds; S. Oikarinen; S. Ramos-Onsins; B. Stranger; P. Van Tienderen; O. Savolainen

doi:10.1046/j.1471-8286.2002.00210.x

Primers for 22 candidate genes for ecological adaptations in Brassicaceae

H. Kuittinen^*, M. Aguadé, D. Charlesworth, A. D.E. Haan, B. Lauga, T. Mitchell-Olds, S. Oikarinen, S. Ramos-Onsins, B. Stranger, P. Van Tienderen, O. Savolainen

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Review article › peer-review

28 Scopus citations

Abstract

There is an increasing interest in direct screening of polymorphisms at candidate loci to associate them with adaptations in natural situations. We report primers that amplify regions at 22 putatively orthologous functional loci in the family Brassicaceae: Arabidopsis thaliana, its two wild outcrossing relatives, and Brassica oleracea. Four groups of genes were known to have specific functions in the model species A. thaliana in ecologically important traits: timing of reproduction; metabolism of secondary compounds; defence against pathogens or light perception. In addition, genes of a fifth group were selected with no consideration of their function, as reference loci.

Original language	English (US)
Pages (from-to)	258-262
Number of pages	5
Journal	Molecular Ecology Notes
Volume	2
Issue number	3
DOIs	https://doi.org/10.1046/j.1471-8286.2002.00210.x
State	Published - Sep 2002

Keywords

Arabidopsis
Candidate genes
Defence-genes
Flowering time
Molecular adaptation
Phenylpropanoid pathway
Phytochromes

ASJC Scopus subject areas

Biochemistry
Ecology
Biochemistry, Genetics and Molecular Biology(all)

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10.1046/j.1471-8286.2002.00210.x

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abstract = "There is an increasing interest in direct screening of polymorphisms at candidate loci to associate them with adaptations in natural situations. We report primers that amplify regions at 22 putatively orthologous functional loci in the family Brassicaceae: Arabidopsis thaliana, its two wild outcrossing relatives, and Brassica oleracea. Four groups of genes were known to have specific functions in the model species A. thaliana in ecologically important traits: timing of reproduction; metabolism of secondary compounds; defence against pathogens or light perception. In addition, genes of a fifth group were selected with no consideration of their function, as reference loci.",

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AU - Kuittinen, H.

AU - Aguadé, M.

AU - Charlesworth, D.

AU - Haan, A. D.E.

AU - Lauga, B.

AU - Mitchell-Olds, T.

AU - Oikarinen, S.

AU - Ramos-Onsins, S.

AU - Stranger, B.

AU - Van Tienderen, P.

AU - Savolainen, O.

PY - 2002/9

Y1 - 2002/9

N2 - There is an increasing interest in direct screening of polymorphisms at candidate loci to associate them with adaptations in natural situations. We report primers that amplify regions at 22 putatively orthologous functional loci in the family Brassicaceae: Arabidopsis thaliana, its two wild outcrossing relatives, and Brassica oleracea. Four groups of genes were known to have specific functions in the model species A. thaliana in ecologically important traits: timing of reproduction; metabolism of secondary compounds; defence against pathogens or light perception. In addition, genes of a fifth group were selected with no consideration of their function, as reference loci.

AB - There is an increasing interest in direct screening of polymorphisms at candidate loci to associate them with adaptations in natural situations. We report primers that amplify regions at 22 putatively orthologous functional loci in the family Brassicaceae: Arabidopsis thaliana, its two wild outcrossing relatives, and Brassica oleracea. Four groups of genes were known to have specific functions in the model species A. thaliana in ecologically important traits: timing of reproduction; metabolism of secondary compounds; defence against pathogens or light perception. In addition, genes of a fifth group were selected with no consideration of their function, as reference loci.

KW - Arabidopsis

KW - Candidate genes

KW - Defence-genes

KW - Flowering time

KW - Molecular adaptation

KW - Phenylpropanoid pathway

KW - Phytochromes

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DO - 10.1046/j.1471-8286.2002.00210.x

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JO - Molecular Ecology Notes

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Primers for 22 candidate genes for ecological adaptations in Brassicaceae

Abstract

Keywords

ASJC Scopus subject areas

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