Principles of E-cadherin supramolecular organization in vivo

Binh An Truong Quang, Madhav Mani, Olga Markova, Thomas Lecuit, Pierre François Lenne*

*Corresponding author for this work

Research output: Contribution to journalArticle

90 Scopus citations

Abstract

Background E-cadherin plays a pivotal role in tissue morphogenesis by forming clusters that support intercellular adhesion and transmit tension. What controls E-cadherin mesoscopic organization in clusters is unclear. Results We use 3D superresolution quantitative microscopy in Drosophila embryos to characterize the size distribution of E-cadherin nanometric clusters. The cluster size follows power-law distributions over three orders of magnitude with exponential decay at large cluster sizes. By exploring the predictions of a general theoretical framework including cluster fusion and fission events and recycling of E-cadherin, we identify two distinct active mechanisms setting the cluster-size distribution. Dynamin-dependent endocytosis targets large clusters only, thereby imposing a cutoff size. Moreover, interactions between E-cadherin clusters and actin filaments control the fission in a size-dependent manner. Conclusions E-cadherin clustering depends on key cortical regulators, which provide tunable and local control over E-cadherin organization. Our data provide the foundation for a quantitative understanding of how E-cadherin distribution affects adhesion and might regulate force transmission in vivo.

Original languageEnglish (US)
Pages (from-to)2197-2207
Number of pages11
JournalCurrent Biology
Volume23
Issue number22
DOIs
StatePublished - Nov 18 2013

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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    Truong Quang, B. A., Mani, M., Markova, O., Lecuit, T., & Lenne, P. F. (2013). Principles of E-cadherin supramolecular organization in vivo. Current Biology, 23(22), 2197-2207. https://doi.org/10.1016/j.cub.2013.09.015