Prismatic Deflection of Live Tumor Cells and Cell Clusters

Peter M. Aldridge, Monorina Mukhopadhyay, Sharif U. Ahmed, Wendi Zhou, Elisa Christinck, Rhema Makonnen, Edward H. Sargent, Shana O. Kelley*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The analysis of heterogeneous subpopulations of circulating tumor cells (CTCs) is critical to enhance our understanding of cancer metastasis and enable noninvasive cancer diagnosis and monitoring. The phenotypic variability and plasticity of these cells - properties closely linked to their clinical behavior - demand techniques that isolate viable, discrete fractions of tumor cells for functional assays of their behavior and detailed analysis of biochemical properties. Here, we introduce the Prism Chip, a high-resolution immunomagnetic profiling and separation chip which harnesses a cobalt-based alloy to separate a flowing stream of nanoparticle-bound tumor cells with differential magnetic loading into 10 discrete streams. Using this approach, we achieve exceptional purity (5.7 log white blood cell depletion) of isolated cells. We test the differential profiling function of the integrated device using prostate cancer blood samples from a mouse xenograft model. Using integrated graphene Hall sensors, we demonstrate concurrent automated profiling of single cells and CTC clusters that belong to distinct subpopulations based on protein surface expression.

Original languageEnglish (US)
Pages (from-to)12692-12700
Number of pages9
JournalACS nano
Volume12
Issue number12
DOIs
StatePublished - Dec 26 2018
Externally publishedYes

Keywords

  • cell analysis
  • circulating tumor cell
  • CTC cluster
  • Hall sensor
  • phenotypic profiling
  • prismatic deflection

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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