Probing protein-carbohydrate interactions with microarrays of synthetic oligosaccharides

Daniel M. Ratner; Eddie W. Adams; Jing Su; Barry R. O'Keefe; Milan Mrksich; Peter H. Seeberger

doi:10.1002/cbic.200300804

Probing protein-carbohydrate interactions with microarrays of synthetic oligosaccharides

Daniel M. Ratner, Eddie W. Adams, Jing Su, Barry R. O'Keefe, Milan Mrksich, Peter H. Seeberger^*

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

179 Scopus citations

Abstract

On the spot: Carbohydrate microarrays, in concert with surface plasmon resonance, are valuable tools in the rapid identification of carbohydrate - protein interactions, like those between high-mannose oligosaccharides and cyanovirin-N (see picture). By utilizing chemical methods amenable to current high-throughput screening technologies, high-density carbohydrate micro-arrays can be prepared and screened by using conventional and widely accessible DNA microarray screening instrumentation.

Original language	English (US)
Pages (from-to)	379-383
Number of pages	5
Journal	ChemBioChem
Volume	5
Issue number	3
DOIs	https://doi.org/10.1002/cbic.200300804
State	Published - Mar 5 2004

Keywords

Carbohydrate chips
Carbohydrates
Lectins
Microarrays
Proteins

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology
Biochemistry
Organic Chemistry

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10.1002/cbic.200300804

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abstract = "On the spot: Carbohydrate microarrays, in concert with surface plasmon resonance, are valuable tools in the rapid identification of carbohydrate - protein interactions, like those between high-mannose oligosaccharides and cyanovirin-N (see picture). By utilizing chemical methods amenable to current high-throughput screening technologies, high-density carbohydrate micro-arrays can be prepared and screened by using conventional and widely accessible DNA microarray screening instrumentation.",

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AU - Su, Jing

AU - O'Keefe, Barry R.

AU - Mrksich, Milan

AU - Seeberger, Peter H.

PY - 2004/3/5

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AB - On the spot: Carbohydrate microarrays, in concert with surface plasmon resonance, are valuable tools in the rapid identification of carbohydrate - protein interactions, like those between high-mannose oligosaccharides and cyanovirin-N (see picture). By utilizing chemical methods amenable to current high-throughput screening technologies, high-density carbohydrate micro-arrays can be prepared and screened by using conventional and widely accessible DNA microarray screening instrumentation.

KW - Carbohydrate chips

KW - Carbohydrates

KW - Lectins

KW - Microarrays

KW - Proteins

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Probing protein-carbohydrate interactions with microarrays of synthetic oligosaccharides

Abstract

Keywords

ASJC Scopus subject areas

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