Processing Bone Marrow with a Semiautomated Cell Processor for Pediatric Transplants: A Comparison of Two Buffy Coat Preparation Methods

Morris Kletzel, Marie L. Olszewski, George F. Dunn*

*Corresponding author for this work

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Thirty-one marrows were harvested from 29 patients and donors in the first year after startup of a six-bed pediatric bone marrow transplant unit at Children's Memorial Hospital in Chicago. Twenty-seven patients were infused, 13 with allogeneic marrow. Eight of the 14 autologous marrows were purged with 4HC. This paper presents cell processing results from the first year of operation, describes a yield-enhancing change made to the buffy coat procedure, and shows which of various parameters significantly influenced final cell recoveries.

Original languageEnglish (US)
Pages (from-to)59-64
Number of pages6
JournalJournal of Hematotherapy
Volume3
Issue number1
DOIs
StatePublished - Jan 1 1994

ASJC Scopus subject areas

  • Immunology
  • Hematology

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