Processing complex mixtures of yeast proteins for direct fragmentation in a quadrupole-FTMS hybrid

Fanyu Meng; Benjamin J. Cargile; Steven M. Patrie; Jeffrey R. Johnson; Thomas N. Corso; Shaun M. Mcloughlin; Neil L. Kelleher

Processing complex mixtures of yeast proteins for direct fragmentation in a quadrupole-FTMS hybrid

Fanyu Meng, Benjamin J. Cargile, Steven M. Patrie, Jeffrey R. Johnson, Thomas N. Corso, Shaun M. Mcloughlin, Neil L. Kelleher

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Analysis of yeast proteins for direct fragmentation in a quadrupole fourier transform mass spectrometry (FTMS) was discussed. Yeast ribosomal proteins with 100% sequence coverage were also analyzed to determine percent occupancy for known post-translational modifications (PTM). S. cerevisiae cells were grown to mid-log phase in YDP media and whole cell extract and ribosomal fractions were obtained following standard procedures. Results show that the method is capable of identifying proteins with 100% sequence coverage at both intact molecular and fragment levels.

Original language	English (US)
Title of host publication	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages	49-50
Number of pages	2
State	Published - Dec 1 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

Cite this

@inproceedings{fae847eb66fd4b91a1cfbff4b42a2045,

title = "Processing complex mixtures of yeast proteins for direct fragmentation in a quadrupole-FTMS hybrid",

abstract = "Analysis of yeast proteins for direct fragmentation in a quadrupole fourier transform mass spectrometry (FTMS) was discussed. Yeast ribosomal proteins with 100% sequence coverage were also analyzed to determine percent occupancy for known post-translational modifications (PTM). S. cerevisiae cells were grown to mid-log phase in YDP media and whole cell extract and ribosomal fractions were obtained following standard procedures. Results show that the method is capable of identifying proteins with 100% sequence coverage at both intact molecular and fragment levels.",

author = "Fanyu Meng and Cargile, {Benjamin J.} and Patrie, {Steven M.} and Johnson, {Jeffrey R.} and Corso, {Thomas N.} and Mcloughlin, {Shaun M.} and Kelleher, {Neil L.}",

year = "2002",

month = dec,

day = "1",

language = "English (US)",

pages = "49--50",

booktitle = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

Meng, F, Cargile, BJ, Patrie, SM, Johnson, JR, Corso, TN, Mcloughlin, SM & Kelleher, NL 2002, Processing complex mixtures of yeast proteins for direct fragmentation in a quadrupole-FTMS hybrid. in Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 49-50, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.

TY - GEN

T1 - Processing complex mixtures of yeast proteins for direct fragmentation in a quadrupole-FTMS hybrid

AU - Meng, Fanyu

AU - Cargile, Benjamin J.

AU - Patrie, Steven M.

AU - Johnson, Jeffrey R.

AU - Corso, Thomas N.

AU - Mcloughlin, Shaun M.

AU - Kelleher, Neil L.

PY - 2002/12/1

Y1 - 2002/12/1

N2 - Analysis of yeast proteins for direct fragmentation in a quadrupole fourier transform mass spectrometry (FTMS) was discussed. Yeast ribosomal proteins with 100% sequence coverage were also analyzed to determine percent occupancy for known post-translational modifications (PTM). S. cerevisiae cells were grown to mid-log phase in YDP media and whole cell extract and ribosomal fractions were obtained following standard procedures. Results show that the method is capable of identifying proteins with 100% sequence coverage at both intact molecular and fragment levels.

AB - Analysis of yeast proteins for direct fragmentation in a quadrupole fourier transform mass spectrometry (FTMS) was discussed. Yeast ribosomal proteins with 100% sequence coverage were also analyzed to determine percent occupancy for known post-translational modifications (PTM). S. cerevisiae cells were grown to mid-log phase in YDP media and whole cell extract and ribosomal fractions were obtained following standard procedures. Results show that the method is capable of identifying proteins with 100% sequence coverage at both intact molecular and fragment levels.

UR - http://www.scopus.com/inward/record.url?scp=2442685752&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442685752&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:2442685752

SP - 49

EP - 50

BT - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Processing complex mixtures of yeast proteins for direct fragmentation in a quadrupole-FTMS hybrid

Abstract

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ASJC Scopus subject areas

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