Production and characterization of a monoclonal antibody to a major concanavalin A-nonbinding antigen of Aspergillus fumigatus

Anoopa Kumar, Viswanath P. Kurup*, Paul A. Greenberger, Jordan N. Fink

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

A hybridoma secreting immunoglobulin G1 antibody was produced against Aspergillus fumigatus. In this study we used interleukin-4 as a growth factor to augment the survival and multiplication of immunoglobulin G1-secreting hybridomas. The antigen recognized by this monoclonal antibody is a low-molecular weight concanavalln A-nonbinding component of A. fumigatus. The monoclonal antibody affinity-purified antigen showed specific reactivity with serum from patients with allergic bronchopulmonary aspergillosis, whereas all allergic asthmatic patients with skin-test reactivity to A. fumigatus demonstrated only low levels of antibodies similar to those in normal control subjects. This antigen failed to show cross-reactivity with other fungal antigens. These results indicate that this monoclonal antibody can be used for the purification of specific antigens useful in the immunodiagnosis of allergic bronchopulmonary aspergillosis.

Original languageEnglish (US)
Pages (from-to)431-436
Number of pages6
JournalThe Journal of laboratory and clinical medicine
Volume121
Issue number3
StatePublished - Mar 1993

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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