Production and characterization of a monoclonal antibody to chicken type I collagen

T. F. Linsenmayer, M. J.C. Hendrix, C. D. Little

Research output: Contribution to journalArticle

57 Scopus citations

Abstract

We have shown that lymphocyte-myeloma cell hybridization can be used to produce large amounts of extremely high-titer specific antibodies against type I collagen, a macromolecule normally of low immunogenicity. In a passive hemagglutination assay the antibody had a high titer against chicken type I collagen but showed no activity against chicken type II or rat type I collagen. By using a two-step fluorescence histochemical procedure on sections of embryonic chicken tibia, strong fluorescence was observed in the perichondrium and surrounding connective tissue (known to contain type I collagen) but not over the cartilage (characterized by type II collagen). When used n conjuction with Staphylococcus aureus as a solid phase immunoadsorbant, the antibody was shown to bind to labeled collagen synthesized in vitro by embryonic chicken calvaria.

Original languageEnglish (US)
Pages (from-to)3703-3707
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume76
Issue number8
DOIs
StatePublished - Jan 1 1979

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