Production of catecholamines in the human epidermis

Karin U. Schallreuter; John M. Wood; Regina Lemke; Caroline LePoole; Pranab Das; Wiete Westerhof; Mark R. Pittelkow; Anthony J. Thody

doi:10.1016/0006-291X(92)91527-W

Production of catecholamines in the human epidermis

Karin U. Schallreuter^*, John M. Wood, Regina Lemke, Caroline LePoole, Pranab Das, Wiete Westerhof, Mark R. Pittelkow, Anthony J. Thody

^*Corresponding author for this work

Dermatology

Research output: Contribution to journal › Article › peer-review

107 Scopus citations

Abstract

Cell-free extracts from human full thickness skin (i.e., epidermis and dermis), suction blister roofs (i.e., epidermis) and from human keratinocytes express biopterin-dependent tyrosine hydroxylase a well as phenylethanolamine-N-methyl transferase, both representing key enzymes for the biosynthesis of epinephrine. These enzyme activities could not be detected in cell extracts from human melanocytes and human fibroblasts. Since keratinocytes in the human epidermis, and in cell cultures, express a high density of beta-2-adrenoceptors, and this signal transduction system regulates intracellular calcium homeostasis, it can be concluded that epinephrine production in the epidermis activates calcium transport via the beta-2-adrenoceptor system. Our results show for the first time that the human epidermis has the capacity to independently produce epinephrine.

Original language	English (US)
Pages (from-to)	72-78
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	189
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(92)91527-W
State	Published - Nov 30 1992

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Production of catecholamines in the human epidermis",

abstract = "Cell-free extracts from human full thickness skin (i.e., epidermis and dermis), suction blister roofs (i.e., epidermis) and from human keratinocytes express biopterin-dependent tyrosine hydroxylase a well as phenylethanolamine-N-methyl transferase, both representing key enzymes for the biosynthesis of epinephrine. These enzyme activities could not be detected in cell extracts from human melanocytes and human fibroblasts. Since keratinocytes in the human epidermis, and in cell cultures, express a high density of beta-2-adrenoceptors, and this signal transduction system regulates intracellular calcium homeostasis, it can be concluded that epinephrine production in the epidermis activates calcium transport via the beta-2-adrenoceptor system. Our results show for the first time that the human epidermis has the capacity to independently produce epinephrine.",

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T1 - Production of catecholamines in the human epidermis

AU - Schallreuter, Karin U.

AU - Wood, John M.

AU - Lemke, Regina

AU - LePoole, Caroline

AU - Das, Pranab

AU - Westerhof, Wiete

AU - Pittelkow, Mark R.

AU - Thody, Anthony J.

PY - 1992/11/30

Y1 - 1992/11/30

N2 - Cell-free extracts from human full thickness skin (i.e., epidermis and dermis), suction blister roofs (i.e., epidermis) and from human keratinocytes express biopterin-dependent tyrosine hydroxylase a well as phenylethanolamine-N-methyl transferase, both representing key enzymes for the biosynthesis of epinephrine. These enzyme activities could not be detected in cell extracts from human melanocytes and human fibroblasts. Since keratinocytes in the human epidermis, and in cell cultures, express a high density of beta-2-adrenoceptors, and this signal transduction system regulates intracellular calcium homeostasis, it can be concluded that epinephrine production in the epidermis activates calcium transport via the beta-2-adrenoceptor system. Our results show for the first time that the human epidermis has the capacity to independently produce epinephrine.

AB - Cell-free extracts from human full thickness skin (i.e., epidermis and dermis), suction blister roofs (i.e., epidermis) and from human keratinocytes express biopterin-dependent tyrosine hydroxylase a well as phenylethanolamine-N-methyl transferase, both representing key enzymes for the biosynthesis of epinephrine. These enzyme activities could not be detected in cell extracts from human melanocytes and human fibroblasts. Since keratinocytes in the human epidermis, and in cell cultures, express a high density of beta-2-adrenoceptors, and this signal transduction system regulates intracellular calcium homeostasis, it can be concluded that epinephrine production in the epidermis activates calcium transport via the beta-2-adrenoceptor system. Our results show for the first time that the human epidermis has the capacity to independently produce epinephrine.

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Production of catecholamines in the human epidermis

Abstract

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