Production of gamma interferon by human T and null cells and its regulation by macrophages

Timothy L. Ratliff*, Richard P. MacDermott, Norma J. Poepping, Dennis M. Oakley, Amos Shapiro, William J. Catalona

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Human mononuclear subpopulations were tested for the capacity to produce interferon after mitogenic stimulation with protein A from Staphylococcus aureus. Mononuclear cells were separated into highly enriched macrophage, T-, B-, and null-cell subpopulations by Sephadex G-10 adherence, anti-human IgG F(ab′) two-column chromatography, and rosetting with sheep erythrocytes. Interferon (IFN) production was observed in both T- and null-cell preparations, but not in macrophage or B-cell preparations. Physicochemical and antigenic characterization of IFN from T- and null-cell preparations showed that both mononuclear subpopulations produced gamma IFN (IFNγ). Regulatory studies showed that IFNγ production was differentially regulated by macrophages. Macrophage addition to T lymphocytes augmented both cellular proliferation and IFNγ production, whereas macrophage addition to null cells suppressed IFNγ production and had no effect on the minimal proliferative response observed for these cells.

Original languageEnglish (US)
Pages (from-to)111-119
Number of pages9
JournalCellular Immunology
Volume74
Issue number1
DOIs
StatePublished - Nov 15 1982

ASJC Scopus subject areas

  • Immunology

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