Poluchenie glitserinizirovannykh modeleǐ myshechnykh volokon s fosforilirovannymi legkimi tsepiami miozina.

Translated title of the contribution: Production of glycerinated models of muscle fibers with phosphorylated myosin light chains

N. N. Lebedeva*, M. Wrotek, L. A. Shuvalova, I. Konkol, I. S. Borovikov

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

A simple method for obtaining glycerinated muscle fibres of m. psoas of rabbit containing regulatory myosin light chains (LC2) of different levels of phosphorylation. The glycerination conditions stimulated endogenic kinase LC2 or phosphatase LC2. Glycerinated muscle fibres contained phosphorylated and dephosphorylated (levels of phosphorylation are 95 +/- 5%, and 5 +/- 5%, respectively) LC2 of myosin. To determine the level of phosphorylation the method of polyacrylamide gel electrophoresis in 8 M urea was modified.

Translated title of the contributionProduction of glycerinated models of muscle fibers with phosphorylated myosin light chains
Original languageRussian
Pages (from-to)973-975
Number of pages3
JournalTsitologiya
Volume29
Issue number8
StatePublished - Aug 1 1987

ASJC Scopus subject areas

  • Histology

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