Poluchenie glitserinizirovannykh modeleǐ myshechnykh volokon s fosforilirovannymi legkimi tsepiami miozina.

N. N. Lebedeva; M. Wrotek; L. A. Shuvalova; I. Konkol; I. S. Borovikov

Poluchenie glitserinizirovannykh modeleǐ myshechnykh volokon s fosforilirovannymi legkimi tsepiami miozina.

Translated title of the contribution: Production of glycerinated models of muscle fibers with phosphorylated myosin light chains

N. N. Lebedeva^*, M. Wrotek, L. A. Shuvalova, I. Konkol, I. S. Borovikov

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A simple method for obtaining glycerinated muscle fibres of m. psoas of rabbit containing regulatory myosin light chains (LC2) of different levels of phosphorylation. The glycerination conditions stimulated endogenic kinase LC2 or phosphatase LC2. Glycerinated muscle fibres contained phosphorylated and dephosphorylated (levels of phosphorylation are 95 +/- 5%, and 5 +/- 5%, respectively) LC2 of myosin. To determine the level of phosphorylation the method of polyacrylamide gel electrophoresis in 8 M urea was modified.

Translated title of the contribution	Production of glycerinated models of muscle fibers with phosphorylated myosin light chains
Original language	Russian
Pages (from-to)	973-975
Number of pages	3
Journal	Tsitologiya
Volume	29
Issue number	8
State	Published - Aug 1 1987

ASJC Scopus subject areas

Histology

Cite this

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title = "Poluchenie glitserinizirovannykh modeleǐ myshechnykh volokon s fosforilirovannymi legkimi tsepiami miozina.",

abstract = "A simple method for obtaining glycerinated muscle fibres of m. psoas of rabbit containing regulatory myosin light chains (LC2) of different levels of phosphorylation. The glycerination conditions stimulated endogenic kinase LC2 or phosphatase LC2. Glycerinated muscle fibres contained phosphorylated and dephosphorylated (levels of phosphorylation are 95 +/- 5%, and 5 +/- 5%, respectively) LC2 of myosin. To determine the level of phosphorylation the method of polyacrylamide gel electrophoresis in 8 M urea was modified.",

author = "Lebedeva, {N. N.} and M. Wrotek and Shuvalova, {L. A.} and I. Konkol and Borovikov, {I. S.}",

year = "1987",

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language = "Russian",

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AU - Lebedeva, N. N.

AU - Wrotek, M.

AU - Shuvalova, L. A.

AU - Konkol, I.

AU - Borovikov, I. S.

PY - 1987/8/1

Y1 - 1987/8/1

N2 - A simple method for obtaining glycerinated muscle fibres of m. psoas of rabbit containing regulatory myosin light chains (LC2) of different levels of phosphorylation. The glycerination conditions stimulated endogenic kinase LC2 or phosphatase LC2. Glycerinated muscle fibres contained phosphorylated and dephosphorylated (levels of phosphorylation are 95 +/- 5%, and 5 +/- 5%, respectively) LC2 of myosin. To determine the level of phosphorylation the method of polyacrylamide gel electrophoresis in 8 M urea was modified.

AB - A simple method for obtaining glycerinated muscle fibres of m. psoas of rabbit containing regulatory myosin light chains (LC2) of different levels of phosphorylation. The glycerination conditions stimulated endogenic kinase LC2 or phosphatase LC2. Glycerinated muscle fibres contained phosphorylated and dephosphorylated (levels of phosphorylation are 95 +/- 5%, and 5 +/- 5%, respectively) LC2 of myosin. To determine the level of phosphorylation the method of polyacrylamide gel electrophoresis in 8 M urea was modified.

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IS - 8

ER -

Poluchenie glitserinizirovannykh modeleǐ myshechnykh volokon s fosforilirovannymi legkimi tsepiami miozina.

Abstract

ASJC Scopus subject areas

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