Production of hydroxyeicosatetraenoic acids and prostaglandins by a novel rat microglial cell line

David J. Rademacher, Christopher S. Kearn, Erica J. Carrier, Sachin Patel, Maria A. Delgado, Andrew Barkmeier, David E. Klick, Nicole M. Breese, Sandra L. Pfister, Kasem Nithipatikom, William B. Campbell, Cecilia J. Hillard*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

We have established a clonal cell line derived from rat microglia that proliferates in response to macrophage-colony stimulating factor (CSF-1). Like primary neonatal microglia, these cells (named RTMGL1) exhibit a ramified morphology, bind isolectin B4, express CD68 and are weakly positive for CD11b and MHC class II. CSF-1-dependent proliferation requires intact signal transduction through several pathways. RTMGL1 synthesize multiple cyclooxygenase (COX) products including 11- and 15-hydroxyeicosatetraenoic acid (HETE) and express COX-2. RTMGL1 synthesize 5-HETE from arachidonic acid (AA) likely via a 5-lipoxygenase (LO). Thus, RTMGL1 have morphological and histological characteristics of primary microglia and metabolize AA via both COX and LO pathways.

Original languageEnglish (US)
Pages (from-to)130-141
Number of pages12
JournalJournal of Neuroimmunology
Volume149
Issue number1-2
DOIs
StatePublished - Apr 2004
Externally publishedYes

Keywords

  • Arachidonic acid
  • CSF-1 receptor
  • Cyclooxygenase
  • Lipoxygenase
  • Macrophage-colony stimulating factor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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