Production of large amounts of 35S RNA and complementary DNA from avian RNA tumor viruses

R. E. Smith*, S. Nebes, J. Leis

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

An automatic perfusion device was used to collect virus-containing supernatant fluids from transformed and untransformed chick embryo fibroblasts every 2 hr. A 30-day perfusion of 10 roller culture bottles of Rous sarcoma virus-infected cells yielded 520 mg of virus and 18 × 109 transformed cells. Quantitative recovery of 60S and 35S RNA required proteinase K treatment prior to phenol deproteinization. Large amounts of complementary DNA were prepared from a nontransforming virus, derived from avian myeloblastosis virus, by using endogenous reverse transcriptase and proteinase K digestion.

Original languageEnglish (US)
Pages (from-to)226-234
Number of pages9
JournalAnalytical Biochemistry
Volume77
Issue number1
DOIs
StatePublished - Jan 1977

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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