PRODUCTION OF MONOCLONAL ANTIBODIES REACTIVE AGAINST CHOLINE ACETYLTRANSFERASE AND THEIR APPLICATION FOR STUDYING CHOLINERGIC SYSTEMS.

Bruce H. Wainer*, Allan I. Levey, Elliott J. Mufson, M. Marsel Mesulam

*Corresponding author for this work

Research output: Book/ReportBook

Abstract

This paper has described experimental approaches for the development and application of monclonal antibodies against the specific cholinergic marker, choline acetyltransferase. Since ChAT was a difficult enzyme to purify, a strategy was developed for detecting the presence of specific antibodies when impure antigen preparations were used for immunization. The strategy included the use of a primary antigen binding assay, double-antibody co-precipitation, to measure antibody, and a radiochemical assay of enzyme activity to detect the presence of specific antigen. Hybridoma technology was then utilized to isolate and propagate clones of lymphoid cells producing antibodies of the desired specificity. Our experience exemplifies the advantages of monoclonal antibody approaches in that pure antigen preparations are not necessary as long as a suitably specific screening procedure is devised. Secondly, once desired clones are isolated, the availablity of continued antibody supplies is theoretically limitless.

Original languageEnglish
PublisherTechnomic Publ Co
Number of pages13
ISBN (Print)0877623910
StatePublished - 1985

Fingerprint

Monoclonal antibodies
Antibodies
Antigens
Assays
Immunization
Enzyme activity
Coprecipitation
Choline
Screening
Enzymes

ASJC Scopus subject areas

  • Engineering(all)

Cite this

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title = "PRODUCTION OF MONOCLONAL ANTIBODIES REACTIVE AGAINST CHOLINE ACETYLTRANSFERASE AND THEIR APPLICATION FOR STUDYING CHOLINERGIC SYSTEMS.",
abstract = "This paper has described experimental approaches for the development and application of monclonal antibodies against the specific cholinergic marker, choline acetyltransferase. Since ChAT was a difficult enzyme to purify, a strategy was developed for detecting the presence of specific antibodies when impure antigen preparations were used for immunization. The strategy included the use of a primary antigen binding assay, double-antibody co-precipitation, to measure antibody, and a radiochemical assay of enzyme activity to detect the presence of specific antigen. Hybridoma technology was then utilized to isolate and propagate clones of lymphoid cells producing antibodies of the desired specificity. Our experience exemplifies the advantages of monoclonal antibody approaches in that pure antigen preparations are not necessary as long as a suitably specific screening procedure is devised. Secondly, once desired clones are isolated, the availablity of continued antibody supplies is theoretically limitless.",
author = "Wainer, {Bruce H.} and Levey, {Allan I.} and Mufson, {Elliott J.} and Mesulam, {M. Marsel}",
year = "1985",
language = "English",
isbn = "0877623910",
publisher = "Technomic Publ Co",

}

PRODUCTION OF MONOCLONAL ANTIBODIES REACTIVE AGAINST CHOLINE ACETYLTRANSFERASE AND THEIR APPLICATION FOR STUDYING CHOLINERGIC SYSTEMS. / Wainer, Bruce H.; Levey, Allan I.; Mufson, Elliott J.; Mesulam, M. Marsel.

Technomic Publ Co, 1985. 13 p.

Research output: Book/ReportBook

TY - BOOK

T1 - PRODUCTION OF MONOCLONAL ANTIBODIES REACTIVE AGAINST CHOLINE ACETYLTRANSFERASE AND THEIR APPLICATION FOR STUDYING CHOLINERGIC SYSTEMS.

AU - Wainer, Bruce H.

AU - Levey, Allan I.

AU - Mufson, Elliott J.

AU - Mesulam, M. Marsel

PY - 1985

Y1 - 1985

N2 - This paper has described experimental approaches for the development and application of monclonal antibodies against the specific cholinergic marker, choline acetyltransferase. Since ChAT was a difficult enzyme to purify, a strategy was developed for detecting the presence of specific antibodies when impure antigen preparations were used for immunization. The strategy included the use of a primary antigen binding assay, double-antibody co-precipitation, to measure antibody, and a radiochemical assay of enzyme activity to detect the presence of specific antigen. Hybridoma technology was then utilized to isolate and propagate clones of lymphoid cells producing antibodies of the desired specificity. Our experience exemplifies the advantages of monoclonal antibody approaches in that pure antigen preparations are not necessary as long as a suitably specific screening procedure is devised. Secondly, once desired clones are isolated, the availablity of continued antibody supplies is theoretically limitless.

AB - This paper has described experimental approaches for the development and application of monclonal antibodies against the specific cholinergic marker, choline acetyltransferase. Since ChAT was a difficult enzyme to purify, a strategy was developed for detecting the presence of specific antibodies when impure antigen preparations were used for immunization. The strategy included the use of a primary antigen binding assay, double-antibody co-precipitation, to measure antibody, and a radiochemical assay of enzyme activity to detect the presence of specific antigen. Hybridoma technology was then utilized to isolate and propagate clones of lymphoid cells producing antibodies of the desired specificity. Our experience exemplifies the advantages of monoclonal antibody approaches in that pure antigen preparations are not necessary as long as a suitably specific screening procedure is devised. Secondly, once desired clones are isolated, the availablity of continued antibody supplies is theoretically limitless.

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