Profile matching and profile subtraction: application of computer-based image analysis system for two-dimensional electrophoresis gels.

C. Lee; E. R. Sherwood; J. A. Sensibar; L. A. Berg; Y. C. Chen; C. C. Tseng

Profile matching and profile subtraction: application of computer-based image analysis system for two-dimensional electrophoresis gels.

C. Lee^*, E. R. Sherwood, J. A. Sensibar, L. A. Berg, Y. C. Chen, C. C. Tseng

^*Corresponding author for this work

Urology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The microcomputer-based image analysis system IB-1000 (developed by Indiana Biotech, Highland, IN) for two-dimensional electrophoresis gels has been described previously (9). It allows the user to compare protein spots between two profiles and identify those spots that are commonly shared in both profiles. This report describes two applications of the system's global comparison routine-profile matching and profile subtraction. This application is able to subtract commonly shared spots from one profile, creating a new profile made up by the unmatched spots in the other profile. These applications can be employed in a large variety of research projects.

Original language	English (US)
Pages (from-to)	374-378
Number of pages	5
Journal	BioTechniques
Volume	7
Issue number	4
State	Published - Apr 1989

Funding

ASJC Scopus subject areas

Biotechnology
General Biochemistry, Genetics and Molecular Biology

Cite this

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title = "Profile matching and profile subtraction: application of computer-based image analysis system for two-dimensional electrophoresis gels.",

abstract = "The microcomputer-based image analysis system IB-1000 (developed by Indiana Biotech, Highland, IN) for two-dimensional electrophoresis gels has been described previously (9). It allows the user to compare protein spots between two profiles and identify those spots that are commonly shared in both profiles. This report describes two applications of the system's global comparison routine-profile matching and profile subtraction. This application is able to subtract commonly shared spots from one profile, creating a new profile made up by the unmatched spots in the other profile. These applications can be employed in a large variety of research projects.",

author = "C. Lee and Sherwood, {E. R.} and Sensibar, {J. A.} and Berg, {L. A.} and Chen, {Y. C.} and Tseng, {C. C.}",

year = "1989",

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language = "English (US)",

volume = "7",

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T2 - application of computer-based image analysis system for two-dimensional electrophoresis gels.

AU - Lee, C.

AU - Sherwood, E. R.

AU - Sensibar, J. A.

AU - Berg, L. A.

AU - Chen, Y. C.

AU - Tseng, C. C.

PY - 1989/4

Y1 - 1989/4

N2 - The microcomputer-based image analysis system IB-1000 (developed by Indiana Biotech, Highland, IN) for two-dimensional electrophoresis gels has been described previously (9). It allows the user to compare protein spots between two profiles and identify those spots that are commonly shared in both profiles. This report describes two applications of the system's global comparison routine-profile matching and profile subtraction. This application is able to subtract commonly shared spots from one profile, creating a new profile made up by the unmatched spots in the other profile. These applications can be employed in a large variety of research projects.

AB - The microcomputer-based image analysis system IB-1000 (developed by Indiana Biotech, Highland, IN) for two-dimensional electrophoresis gels has been described previously (9). It allows the user to compare protein spots between two profiles and identify those spots that are commonly shared in both profiles. This report describes two applications of the system's global comparison routine-profile matching and profile subtraction. This application is able to subtract commonly shared spots from one profile, creating a new profile made up by the unmatched spots in the other profile. These applications can be employed in a large variety of research projects.

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ER -

Profile matching and profile subtraction: application of computer-based image analysis system for two-dimensional electrophoresis gels.

Abstract

Funding

ASJC Scopus subject areas

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