Profiling glucose-induced selective inhibition of disaccharide catabolism in Bacillus megaterium QM B1551 by stable isotope labelling

Tracy Youngster, Julie A. Wushensky, Ludmilla Aristilde*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

We investigated the co-catabolism of carbohydrate mixtures in Bacillus megaterium QM B1551 using a 13C-assisted metabolomics profiling approach. Specifically, we monitored the ability of B. megaterium to achieve the simultaneous catabolism of glucose and a common disaccharide –cellobiose, maltose, or sucrose. Growth experiments indicated that each disaccharide alone can serve as a sole carbon source for B. megaterium, in accordance with the genetic analysis of this bacterium, which predicted diverse metabolic capabilities. However, following growth on 13C-labelled glucose and each unlabelled disaccharide, the labelling patterns of the intracellular metabolites in glycolysis and the pentose phosphate pathway revealed a hierarchy in disaccharide catabolism: (i) complete inhibition of cellobiose catabolism, (ii) minimal catabolism of maltose and (iii) unbiased catabolism of sucrose. The labelling of amino acids confirmed this selective assimilation of each substrate in biomass precursors. This study highlights the fact that B. megaterium exhibits a mixedcarbohydrate utilization that is different from that of B. subtilis, the most studied model Bacillus species.

Original languageEnglish (US)
Article number000540
Pages (from-to)1509-1514
Number of pages6
JournalMicrobiology (United Kingdom)
Volume163
Issue number10
DOIs
StatePublished - Oct 2017

Keywords

  • Bacillus
  • Carbohydrate
  • Metabolism
  • Organic carbon decomposition
  • Soil bacteria

ASJC Scopus subject areas

  • Microbiology

Fingerprint Dive into the research topics of 'Profiling glucose-induced selective inhibition of disaccharide catabolism in Bacillus megaterium QM B1551 by stable isotope labelling'. Together they form a unique fingerprint.

Cite this