Profiling of acyl-CoA oxidase-deficient and peroxisome proliferator Wy14,643-treated mouse liver protein by surface-enhanced laser desorption/ionization ProteinChip® biology system

R. Chu*, W. Zhang, H. Lim, A. V. Yeldandi, C. Herring, L. Brumfield, J. K. Reddy, M. Davison

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Peroxisome proliferators induce hepatic peroxasome proliferation and hepatocellular carcinomas in rodents. These chemicals increase the expression of the peroxisomal β-oxidation pathway and the cytochrome P-450 4A family, which metabolizes lipids, including fatty acids. Mice lacking fatty acyl-CoA oxidase (AOX-/-), the first enzyme of the peroxisomal β-oxidation system, exhibit extensive microvesicular steatohepatitis, leading to hepatocellular regeneration and massive peroxisome proliferation. To investigate proteins involved in peroxisome proliferation, we adopted a novel surface-enhanced laser desorption/ionization (SELDI) ProteinChip technology to compare the protein profiles of control (wild-type), AOX-/-, and wild-type mice treated with peroxisome proliferator, Wy-14,643. The results indicated that the protein profiles of AOX-/- mice were similar to the wild-type mice treated with Wy14,643, but significantly different from the nontreated wild-type mice. Using four different ProteinChip Arrays, a total of 40 protein peaks showed more than twofold changes. Among these differentially expressed peaks, a downregulated peak was identified as the major urinary protein in both AOX-/- and Wy14,643-treated mice by SELDI. The identification of MUP was further confirmed by two-dimensional electrophoresis and liquid chromatography coupled tandem mass spectrometry (LC-MS-MS). This SELDI method offers several technical advantages for detection of differentially expressed proteins, including ease and speed of screening, no need for chromatographic processing, and small sample size.

Original languageEnglish (US)
Pages (from-to)165-177
Number of pages13
JournalGene expression
Volume10
Issue number4
DOIs
StatePublished - 2002

Keywords

  • Acyl-CoA oxidase
  • Major urinary protein
  • Mass spectrometry
  • Peroxisome proliferator
  • Peroxisome proliferator-activated receptor
  • ProteinChip Array
  • SELDI

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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