Prognostic Value of Cell-Surface Vimentin-Positive CTCs in Pediatric Sarcomas

Long Dao, Dristhi Ragoonanan, Izhar Batth, Arun Satelli, Jessica Foglesong, Jian Wang, Wafik Zaky, Jonathan B. Gill, Diane Liu, Aisha Albert, Nancy Gordon, Winston Huh, Douglas Harrison, Cynthia Herzog, Eugenie Kleinerman, Richard Gorlick, Najat Daw, Shulin Li*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Background: Despite advances in care, the 5 year overall survival for patients with relapsed and or metastatic sarcoma remains as low as < 35%. Currently, there are no biomarkers available to assess disease status in patients with sarcomas and as such, disease surveillance remains reliant on serial imaging which increases the risk of secondary malignancies and heightens patient anxiety. Methods: Here, for the first time reported in the literature, we have enumerated the cell surface vimentin (CSV+) CTCs in the blood of 92 sarcoma pediatric and adolescent and young adult (AYA) patients as a possible marker of disease. Results: We constructed a ROC with an AUC of 0.831 resulting in a sensitivity of 85.3% and a specificity of 75%. Additionally, patients who were deemed to be CSV+ CTC positive were found to have a worse overall survival compared to those who were CSV+ CTC negative. We additionally found the use of available molecular testing increased the accuracy of our diagnostic and prognostic tests. Conclusions: Our findings indicate that CSV+ CTCs have prognostic value and can possibly serve as a measure of disease burden.

Original languageEnglish (US)
Article number760267
JournalFrontiers in Oncology
StatePublished - Dec 9 2021


  • adult and young adolescent (AYA)
  • area under curve (AUC)
  • cell surface vimentin (CSV)
  • circulating tumor cell (CTC)
  • clinical laboratory improvement amendments (CLIA)
  • epithelial cellular adhesion molecule (EpCAM)
  • receiver operating characteristic curve (ROC)

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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