Programmable fluorogenic enzyme-responsive micellar nanoparticles

M. P. Chien; M. P. Thompson; N. C. Gianneschi

Programmable fluorogenic enzyme-responsive micellar nanoparticles

M. P. Chien, M. P. Thompson, N. C. Gianneschi

Chemistry

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We developed enzyme-responsive fluorogenic micellar nanoparticles with detectable spectrophotometric properties unique to the particles and their aggregated state. These micelles are assembled from peptide-polymer amphiphiles (PPAs) labeled with either fluorescein or rhodamine. This is achieved by labeling otherwise similar block copolymer amphiphiles with each of the dyes. When mixed together, signals from the FRET-pair can be utilized to detect particle assembly and hence enzymatic activity. Furthermore, we show FRET signals within the shell of the assembled micelles can be used to estimate particle stability (critical aggregation concentration). This system is designed to be applied in physiologically relavant settings, having broad implications for the design of nanoscale materials in drug delivery and diagnostics.

Original language	English (US)
Title of host publication	Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
Pages	304-307
Number of pages	4
Volume	3
State	Published - Aug 9 2013
Event	Nanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013 - Washington, DC, United States Duration: May 12 2013 → May 16 2013

Other

Other	Nanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
Country/Territory	United States
City	Washington, DC
Period	5/12/13 → 5/16/13

Keywords

Drug delivery
FRET
Nanoparticles

ASJC Scopus subject areas

Biotechnology

Cite this

@inproceedings{c347c3a4174a4d7e92a8c5a529cd3ccd,

title = "Programmable fluorogenic enzyme-responsive micellar nanoparticles",

abstract = "We developed enzyme-responsive fluorogenic micellar nanoparticles with detectable spectrophotometric properties unique to the particles and their aggregated state. These micelles are assembled from peptide-polymer amphiphiles (PPAs) labeled with either fluorescein or rhodamine. This is achieved by labeling otherwise similar block copolymer amphiphiles with each of the dyes. When mixed together, signals from the FRET-pair can be utilized to detect particle assembly and hence enzymatic activity. Furthermore, we show FRET signals within the shell of the assembled micelles can be used to estimate particle stability (critical aggregation concentration). This system is designed to be applied in physiologically relavant settings, having broad implications for the design of nanoscale materials in drug delivery and diagnostics.",

keywords = "Drug delivery, FRET, Nanoparticles",

author = "Chien, {M. P.} and Thompson, {M. P.} and Gianneschi, {N. C.}",

year = "2013",

month = aug,

day = "9",

language = "English (US)",

isbn = "9781482205862",

volume = "3",

pages = "304--307",

booktitle = "Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013",

note = "Nanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013 ; Conference date: 12-05-2013 Through 16-05-2013",

}

Chien, MP, Thompson, MP & Gianneschi, NC 2013, Programmable fluorogenic enzyme-responsive micellar nanoparticles. in Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013. vol. 3, pp. 304-307, Nanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013, Washington, DC, United States, 5/12/13.

TY - GEN

T1 - Programmable fluorogenic enzyme-responsive micellar nanoparticles

AU - Chien, M. P.

AU - Thompson, M. P.

AU - Gianneschi, N. C.

PY - 2013/8/9

Y1 - 2013/8/9

N2 - We developed enzyme-responsive fluorogenic micellar nanoparticles with detectable spectrophotometric properties unique to the particles and their aggregated state. These micelles are assembled from peptide-polymer amphiphiles (PPAs) labeled with either fluorescein or rhodamine. This is achieved by labeling otherwise similar block copolymer amphiphiles with each of the dyes. When mixed together, signals from the FRET-pair can be utilized to detect particle assembly and hence enzymatic activity. Furthermore, we show FRET signals within the shell of the assembled micelles can be used to estimate particle stability (critical aggregation concentration). This system is designed to be applied in physiologically relavant settings, having broad implications for the design of nanoscale materials in drug delivery and diagnostics.

AB - We developed enzyme-responsive fluorogenic micellar nanoparticles with detectable spectrophotometric properties unique to the particles and their aggregated state. These micelles are assembled from peptide-polymer amphiphiles (PPAs) labeled with either fluorescein or rhodamine. This is achieved by labeling otherwise similar block copolymer amphiphiles with each of the dyes. When mixed together, signals from the FRET-pair can be utilized to detect particle assembly and hence enzymatic activity. Furthermore, we show FRET signals within the shell of the assembled micelles can be used to estimate particle stability (critical aggregation concentration). This system is designed to be applied in physiologically relavant settings, having broad implications for the design of nanoscale materials in drug delivery and diagnostics.

KW - Drug delivery

KW - FRET

KW - Nanoparticles

UR - http://www.scopus.com/inward/record.url?scp=84881095082&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881095082&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84881095082

SN - 9781482205862

VL - 3

SP - 304

EP - 307

BT - Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013

T2 - Nanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013

Y2 - 12 May 2013 through 16 May 2013

ER -

Programmable fluorogenic enzyme-responsive micellar nanoparticles

Abstract

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Keywords

ASJC Scopus subject areas

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