Programmable fluorogenic enzyme-responsive micellar nanoparticles

M. P. Chien, M. P. Thompson, N. C. Gianneschi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We developed enzyme-responsive fluorogenic micellar nanoparticles with detectable spectrophotometric properties unique to the particles and their aggregated state. These micelles are assembled from peptide-polymer amphiphiles (PPAs) labeled with either fluorescein or rhodamine. This is achieved by labeling otherwise similar block copolymer amphiphiles with each of the dyes. When mixed together, signals from the FRET-pair can be utilized to detect particle assembly and hence enzymatic activity. Furthermore, we show FRET signals within the shell of the assembled micelles can be used to estimate particle stability (critical aggregation concentration). This system is designed to be applied in physiologically relavant settings, having broad implications for the design of nanoscale materials in drug delivery and diagnostics.

Original languageEnglish (US)
Title of host publicationTechnical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
Pages304-307
Number of pages4
Volume3
StatePublished - Aug 9 2013
EventNanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013 - Washington, DC, United States
Duration: May 12 2013May 16 2013

Other

OtherNanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
CountryUnited States
CityWashington, DC
Period5/12/135/16/13

Keywords

  • Drug delivery
  • FRET
  • Nanoparticles

ASJC Scopus subject areas

  • Biotechnology

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  • Cite this

    Chien, M. P., Thompson, M. P., & Gianneschi, N. C. (2013). Programmable fluorogenic enzyme-responsive micellar nanoparticles. In Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013 (Vol. 3, pp. 304-307)