Programmed Ribosomal Frameshifting Generates a Copper Transporter and a Copper Chaperone from the Same Gene

Sezen Meydan, Dorota Klepacki, Subbulakshmi Karthikeyan, Tõnu Margus, Paul Thomas, John E. Jones, Yousuf Khan, Joseph Briggs, Jonathan D. Dinman, Nora Vázquez-Laslop*, Alexander S. Mankin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Metal efflux pumps maintain ion homeostasis in the cell. The functions of the transporters are often supported by chaperone proteins, which scavenge the metal ions from the cytoplasm. Although the copper ion transporter CopA has been known in Escherichia coli, no gene for its chaperone had been identified. We show that the CopA chaperone is expressed in E. coli from the same gene that encodes the transporter. Some ribosomes translating copA undergo programmed frameshifting, terminate translation in the −1 frame, and generate the 70 aa-long polypeptide CopA(Z), which helps cells survive toxic copper concentrations. The high efficiency of frameshifting is achieved by the combined stimulatory action of a “slippery” sequence, an mRNA pseudoknot, and the CopA nascent chain. Similar mRNA elements are not only found in the copA genes of other bacteria but are also present in ATP7B, the human homolog of copA, and direct ribosomal frameshifting in vivo.

Original languageEnglish (US)
Pages (from-to)207-219
Number of pages13
JournalMolecular cell
Volume65
Issue number2
DOIs
StatePublished - Jan 19 2017

Keywords

  • copA
  • nascent peptide
  • pseudoknot
  • recoding
  • ribosome profiling
  • slippery sequence
  • translation regulation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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