Proinflammatory and type 1 cytokine expression in cervical mucosa during HIV-1 and human papillomavirus infection

Homira Behbahani*, Lilian Walther-Jallow, Elin Klareskog, Linda Baum, Audrey L. French, Bruce K. Patterson, Patricia Garcia, Anna Lena Spetz, Alan Landay, Jan Andersson

*Corresponding author for this work

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Suppression of immune activation and increased inflammation are prevalent during viral infection. To investigate the role of inflammation in HIV transmission, we studied the infectious and inflammatory milieu in cervical mucosa from HIV-1- and human papillomavirus (HPV)-coinfected and HPV-monoinfected women. The numbers of cytokine-, chemokine-, and p24-expressing cells were determined using in situ imaging analysis and intracellular staining of p24 antigen. Significantly higher expression of the proinflammatory cytokines, interleukin (IL)-1α/β, was seen in cervical tissue from HIV/HPV-coinfected as compared with HPV-monoinfected tissues, whereas IL-2- and interferon (IFN)-γ-expressing cells were higher in HPV-monoinfected tissues. IL-10 was low in both groups, whereas IL-4 was significantly higher in HPV-monoinfected and HIV/HPV-coinfected tissues than in HIV/HPV-negative controls. RANTES and macrophage inflammatory protein (MIP)-1β but not MIP-1α were significantly higher in the genital tract of HIV/HPV-coinfected as compared with HPV-monoinfected individuals and controls. HIV/HPV-coinfected tissues had a higher level of human leukocyte antigen D-related (HLA-DR)-expressing dendritic cells (DCs). There was a positive correlation between the number of CD4 and CD8 T cells as well as CD1a, IL-1α, and RANTES expression and p24 antigen-expressing cells in the HIV/HPV-coinfected tissues. These findings suggest the persistence of immune activation and inflammation in the genital tract of women with HPV monoinfection and in HIV-infected women coinfected with HPV.

Original languageEnglish (US)
Pages (from-to)9-19
Number of pages11
JournalJournal of Acquired Immune Deficiency Syndromes
Volume45
Issue number1
DOIs
StatePublished - May 2007

Keywords

  • Chemokine
  • Cytokine
  • Dendritic cells
  • Genital tract
  • Human papillomavirus
  • In situ imaging

ASJC Scopus subject areas

  • Infectious Diseases
  • Pharmacology (medical)

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    Behbahani, H., Walther-Jallow, L., Klareskog, E., Baum, L., French, A. L., Patterson, B. K., Garcia, P., Spetz, A. L., Landay, A., & Andersson, J. (2007). Proinflammatory and type 1 cytokine expression in cervical mucosa during HIV-1 and human papillomavirus infection. Journal of Acquired Immune Deficiency Syndromes, 45(1), 9-19. https://doi.org/10.1097/QAI.0b013e3180415da7