Proliferation and phenotypic changes of stromal cells in response to varying estrogen/androgen levels in castrated rats

Ying Zhou, Xiang Qian Xiao, Lin Feng Chen, Rui Yang, Jian Dang Shi, Xiao Ling Du, Helmut Klocker, Irwin Park, Chung Lee, Ju Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

It is known that human benign prostatic hyperplasia might arise from an estrogen/androgen (E/T) imbalance. We studied the response of castrated rat prostate to different ratios of circulating E/T. The castrated male Wistar rats were randomly injected with E/T at different ratios for 4 weeks. The prostates of E/T (1:100) group showed a distinct prostatic hyperplasia response by prostatic index, hematoxylin and eosin staining, and quantitative immunohistochemical analysis of α-smooth muscle actin (SMA). In this group, cells positive for Vimentin, non-muscle myosin heavy chain (NMMHC) and proliferating cell nuclear antigen (PCNA) increased in the stroma and epithelium. Furthermore, the mRNA levels of smooth muscle myosin heavy chain (SMMHC) and NMMHC increased. So E/T at a ratio of 1:100 can induce a stromal hyperplastic response in the prostate of castrated rats. The main change observed was an increase of smooth muscle cells, whereas some epithelial changes were also seen in the rat prostates.

Original languageEnglish (US)
Pages (from-to)451-459
Number of pages9
JournalAsian Journal of Andrology
Volume11
Issue number4
DOIs
StatePublished - 2009

ASJC Scopus subject areas

  • Urology

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