Promoting extracellular matrix remodeling via ascorbic acid enhances the survival of primary ovarian follicles encapsulated in alginate hydrogels

David Tagler, Yogeshwar Makanji, Tao Tu, Beatriz Peñalver Bernabé, Raymond Lee, Jie Zhu, Ekaterina Kniazeva, Jessica E. Hornick, Teresa K. Woodruff, Lonnie D. Shea*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

The in vitro growth of ovarian follicles is an emerging technology for fertility preservation. Various strategies support the culture of secondary and multilayer follicles from various species including mice, non-human primate, and human; however, the culture of early stage (primary and primordial) follicles, which are more abundant in the ovary and survive cryopreservation, has been limited. Hydrogel-encapsulating follicle culture systems that employed feeder cells, such as mouse embryonic fibroblasts (MEFs), stimulated the growth of primary follicles (70-80μm); yet, survival was low and smaller follicles (<70μm) rapidly lost structure and degenerated. These morphologic changes were associated with a breakdown of the follicular basement membrane; hence, this study investigated ascorbic acid based on its role in extracellular matrix (ECM) deposition/remodeling for other applications. The selection of ascorbic acid was further supported by a microarray analysis that suggested a decrease in mRNA levels of enzymes within the ascorbate pathway between primordial, primary, and secondary follicles. The supplementation of ascorbic acid (50μg/mL) significantly enhanced the survival of primary follicles (<80μm) cultured in alginate hydrogels, which coincided with improved structural integrity. Follicles developed antral cavities and increased to diameters exceeding 250μm. Consistent with improved structural integrity, the gene/protein expression of ECM and cell adhesion molecules was significantly changed. This research supports the notion that modifying the culture environment (medium components) can substantially enhance the survival and growth of early stage follicles.

Original languageEnglish (US)
Pages (from-to)1417-1429
Number of pages13
JournalBiotechnology and Bioengineering
Volume111
Issue number7
DOIs
StatePublished - Jul 2014

Keywords

  • Alginate hydrogel
  • Ascorbic acid
  • Biomaterial
  • Extracellular matrix
  • Ovarian follicle development
  • Primary follicle
  • Regenerative medicine
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Fingerprint

Dive into the research topics of 'Promoting extracellular matrix remodeling via ascorbic acid enhances the survival of primary ovarian follicles encapsulated in alginate hydrogels'. Together they form a unique fingerprint.

Cite this