TY - JOUR
T1 - Properties of enucleated cells
T2 - II. Characteristic overlapping of transformed cells is reestablished by enucleates
AU - Pollack, R.
AU - Goldman, R. D.
AU - Conlon, S.
AU - Chang, C.
N1 - Funding Information:
This work was supported by grants from the National tute and the National Science Foundation.
PY - 1974/9
Y1 - 1974/9
N2 - Many normal cells are restricted in movement over each other's surfaces. We have quantitatively examined this restriction in the established mouse cell line 3T3, in a polyoma virus-transformed subline Py3T3, and in enucleated cytoplasms prepared from these lines by cytochalasin B treatment. Overlapping of trypsinized, replated cells was quantitated by phase contrast and scanning electron microscopy. Cells of the normal mouse line 3T3 did not overlap one another. Cells of Py3T3 overlapped one another extensively. Trypsinized, replated 3T3 enucleates did not overlap one another, whereas replated enucleates of Py3T3 displayed many overlaps.
AB - Many normal cells are restricted in movement over each other's surfaces. We have quantitatively examined this restriction in the established mouse cell line 3T3, in a polyoma virus-transformed subline Py3T3, and in enucleated cytoplasms prepared from these lines by cytochalasin B treatment. Overlapping of trypsinized, replated cells was quantitated by phase contrast and scanning electron microscopy. Cells of the normal mouse line 3T3 did not overlap one another. Cells of Py3T3 overlapped one another extensively. Trypsinized, replated 3T3 enucleates did not overlap one another, whereas replated enucleates of Py3T3 displayed many overlaps.
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U2 - 10.1016/0092-8674(74)90038-5
DO - 10.1016/0092-8674(74)90038-5
M3 - Article
C2 - 4370294
AN - SCOPUS:0016303206
SN - 0092-8674
VL - 3
SP - 51
EP - 54
JO - Cell
JF - Cell
IS - 1
ER -