Properties of enucleated cells: II. Characteristic overlapping of transformed cells is reestablished by enucleates

R. Pollack*, R. D. Goldman, S. Conlon, C. Chang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Many normal cells are restricted in movement over each other's surfaces. We have quantitatively examined this restriction in the established mouse cell line 3T3, in a polyoma virus-transformed subline Py3T3, and in enucleated cytoplasms prepared from these lines by cytochalasin B treatment. Overlapping of trypsinized, replated cells was quantitated by phase contrast and scanning electron microscopy. Cells of the normal mouse line 3T3 did not overlap one another. Cells of Py3T3 overlapped one another extensively. Trypsinized, replated 3T3 enucleates did not overlap one another, whereas replated enucleates of Py3T3 displayed many overlaps.

Original languageEnglish (US)
Pages (from-to)51-54
Number of pages4
JournalCell
Volume3
Issue number1
DOIs
StatePublished - Sep 1974

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Properties of enucleated cells: II. Characteristic overlapping of transformed cells is reestablished by enucleates'. Together they form a unique fingerprint.

Cite this