ProSight Annotator: Complete control and customization of protein entries in UniProt XML files

Joseph B. Greer, Bryan P. Early, Kenneth R. Durbin, Steven M. Patrie, Paul M. Thomas, Neil L. Kelleher, Richard D. LeDuc, Ryan T. Fellers*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


The effectiveness of any proteomics database search depends on the theoretical candidate information contained in the protein database. Unfortunately, candidate entries from protein databases such as UniProt rarely contain all the post-translational modifications (PTMs), disulfide bonds, or endogenous cleavages of interest to researchers. These omissions can limit discovery of novel and biologically important proteoforms. Conversely, searching for a specific proteoform becomes a computationally difficult task for heavily modified proteins. Both situations require updates to the database through user-annotated entries. Unfortunately, manually creating properly formatted UniProt Extensible Markup Language (XML) files is tedious and prone to errors. ProSight Annotator solves these issues by providing a graphical interface for adding user-defined features to UniProt-formatted XML files for better informed proteoform searches. It can be downloaded from

Original languageEnglish (US)
Article number2100209
Issue number11-12
StatePublished - Jun 2022


  • UniProt
  • bottom-up proteomics
  • post-translational modification
  • proteoforms
  • proteomics software
  • top-down proteomics

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry


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