ProSight Lite: Graphical software to analyze top-down mass spectrometry data

Ryan T. Fellers, Joseph B. Greer, Bryan P. Early, Xiang Yu, Richard D. Leduc, Neil L. Kelleher*, Paul M. Thomas

*Corresponding author for this work

Research output: Contribution to journalArticle

98 Scopus citations

Abstract

Many top-down proteomics experiments focus on identifying and localizing PTMs and other potential sources of "mass shift" on a known protein sequence. A simple application to match ion masses and facilitate the iterative hypothesis testing of PTM presence and location would assist with the data analysis in these experiments. ProSight Lite is a free software tool for matching a single candidate sequence against a set of mass spectrometric observations. Fixed or variable modifications, including both PTMs and a select number of glycosylations, can be applied to the amino acid sequence. The application reports multiple scores and a matching fragment list. Fragmentation maps can be exported for publication in either portable network graphic (PNG) or scalable vector graphic (SVG) format. ProSight Lite can be freely downloaded from http://prosightlite.northwestern.edu, installs and updates from the web, and requires Windows 7 or a higher version.

Original languageEnglish (US)
Pages (from-to)1235-1238
Number of pages4
JournalProteomics
Volume15
Issue number7
DOIs
StatePublished - Apr 1 2015

Keywords

  • Bioinformatics
  • MS analysis
  • Proteoform characterization
  • Proteomics software
  • Top-down proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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