ProSight Lite: Graphical software to analyze top-down mass spectrometry data

Ryan T. Fellers, Joseph B. Greer, Bryan P. Early, Xiang Yu, Richard D. Leduc, Neil L. Kelleher*, Paul M. Thomas

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

116 Scopus citations


Many top-down proteomics experiments focus on identifying and localizing PTMs and other potential sources of "mass shift" on a known protein sequence. A simple application to match ion masses and facilitate the iterative hypothesis testing of PTM presence and location would assist with the data analysis in these experiments. ProSight Lite is a free software tool for matching a single candidate sequence against a set of mass spectrometric observations. Fixed or variable modifications, including both PTMs and a select number of glycosylations, can be applied to the amino acid sequence. The application reports multiple scores and a matching fragment list. Fragmentation maps can be exported for publication in either portable network graphic (PNG) or scalable vector graphic (SVG) format. ProSight Lite can be freely downloaded from, installs and updates from the web, and requires Windows 7 or a higher version.

Original languageEnglish (US)
Pages (from-to)1235-1238
Number of pages4
Issue number7
StatePublished - Apr 1 2015


  • Bioinformatics
  • MS analysis
  • Proteoform characterization
  • Proteomics software
  • Top-down proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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