Protein acetylation dynamics in response to carbon overflow in Escherichia coli

Birgit Schilling, David Christensen, Robert Davis, Alexandria K. Sahu, Linda I. Hu, Arti Walker-Peddakotla, Dylan J. Sorensen, Bozena Zemaitaitis, Bradford W. Gibson, Alan J. Wolfe*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

122 Scopus citations

Abstract

In Escherichia coli, acetylation of proteins at lysines depends largely on a non-enzymatic acetyl phosphate-dependent mechanism. To assess the functional significance of this post-translational modification, we first grew wild-type cells in buffered tryptone broth with glucose and monitored acetylation over time by immunochemistry. Most acetylation occurred in stationary phase and paralleled glucose consumption and acetate excretion, which began upon entry into stationary phase. Transcription of rprA, a stationary phase regulator, exhibited similar behavior. To identify sites and substrates with significant acetylation changes, we used label-free, quantitative proteomics to monitor changes in protein acetylation. During growth, both the number of identified sites and the extent of acetylation increased with considerable variation among lysines from the same protein. As glucose-regulated lysine acetylation was predominant in central metabolic pathways and overlapped with acetyl phosphate-regulated acetylation sites, we deleted the major carbon regulator CRP and observed a dramatic loss of acetylation that could be restored by deleting the enzyme that degrades acetyl phosphate. We propose that acetyl phosphate-dependent acetylation is a response to carbon flux that could regulate central metabolism.

Original languageEnglish (US)
Pages (from-to)847-863
Number of pages17
JournalMolecular Microbiology
Volume98
Issue number5
DOIs
StatePublished - Dec 1 2015

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

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