Protein kinase Darkener of apricot and its substrate EF1γ regulate organelle transport along microtubules

Anna S. Serpinskaya, Karine Tuphile, Leonard Rabinow, Vladimir I. Gelfand*

*Corresponding author for this work

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Regulation of organelle transport along microtubules is important for proper distribution of membrane organelles and protein complexes in the cytoplasm. RNAi-mediated knockdown in cultured Drosophila S2 cells demonstrates that two microtubule-binding proteins, a unique isoform of Darkener of apricot (DOA) protein kinase, and its substrate, translational elongation factor EF1γ, negatively regulate transport of several classes of membrane organelles along microtubules. Inhibition of transport by EF1γ requires its phosphorylation by DOA on serine 294. Together, our results indicate a new role for two proteins that have not previously been implicated in regulation of the cytoskeleton. These results further suggest that the biological role of some of the proteins binding to the microtubule track is to regulate cargo transport along these tracks.

Original languageEnglish (US)
Pages (from-to)33-39
Number of pages7
JournalJournal of cell science
Volume127
Issue number1
DOIs
StatePublished - Jan 1 2014

Keywords

  • DOA Translation elongation factor EF1γ
  • Darkener of apricot
  • Drosophila melanogaster S2 cells
  • Ef1γ
  • Organelle transport

ASJC Scopus subject areas

  • Cell Biology

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