Protein nanoarrays generated by dip-pen nanotithography

Ki Bum Lee, So Jung Park, Chad A. Mirkin, Jennifer C. Smith, Milan Mrksich*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1070 Scopus citations

Abstract

Dip-pen nanolithography was used to construct arrays of proteins with 100- to 350-nanometer features. These nanoarrays exhibit almost no detectable nonspecific binding of proteins to their passivated portions even in complex mixtures of proteins, and therefore provide the opportunity to study a variety of surface-mediated biological recognition processes. For example, reactions involving the protein features and antigens in complex solutions can be screened easily by atomic force microscopy. As further proof-of-concept, these arrays were used to study cellular adhesion at the submicrometer scale.

Original languageEnglish (US)
Pages (from-to)1702-1705
Number of pages4
JournalScience
Volume295
Issue number5560
DOIs
StatePublished - Mar 1 2002

ASJC Scopus subject areas

  • General

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