Protein tyrosine kinase p56lck controls allelic exclusion of T-cell receptor β-chain genes

Steven J. Anderson, Steven D. Levin, Roger M. Perlmutter*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

128 Scopus citations

Abstract

DURING T-cell development, site-specific DNA rearrangements mediating assembly of β- and α-chain genes of the T-cell receptor (TCR) are developmentally ordered1,2. In particular, assembly and expression of a complete β-chain gene blocks further rearrangements at the β-locus (a process referred to as allelic exclusion)3 and drives the generation and expansion of CD4+ 8+ cells4,5. Although the mechanism used by TCRβ chains to deliver such signals is unknown, studies in transgenic animals have suggested that the lymphocyte-specific protein tyrosine kinase p56lck may impinge on a similar signalling pathway6. The hypothesis that TCRβ chains deliver intracellular signals via p56lck makes an explicit prediction: that interference with p56lck function will mitigate the effects of a simultaneously expressed TCRβ chain. Here we confirm this prediction through examination of allelic exclusion in mice expressing both a functional TCRβ chain transgene and a catalytically inactive form of p56lck.

Original languageEnglish (US)
Pages (from-to)552-554
Number of pages3
JournalNature
Volume365
Issue number6446
DOIs
StatePublished - Jan 1 1993

ASJC Scopus subject areas

  • General

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