Proteins of the rat prostate: I. Preliminary characterization by two‐dimensional electrophoresis

Chung Lee*, Yvonne Tsai, Julia Sensibar, Harold H. Harrison

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The rat prostate consists of three distinct lobes: the ventral, lateral, and dorsal. Proteins in the three lobes of the prostate were studied with the ISO‐DALT system for high‐resolution two‐dimensional electrophoresis. Proteins were detected with ammoniacal silver stain. Comparison of patterns from the three lobes of the prostate of control, noncastrated rats revealed that while there was a remarkable overall similarity, six groups of proteins showed lobe‐specific differences. When prostatic regression was induced by castration, androgen‐dependent proteins showed a decrease in staining intensity. A group of proteins, with pI 5.0–6.0 and MW 65,000–70,000, was consistently observed only during the active phase of prostatic regression (days 3–7 postcastration). Their presence during this specific interval may play a role in tissue involution.

Original languageEnglish (US)
Pages (from-to)171-182
Number of pages12
JournalThe Prostate
Volume7
Issue number2
DOIs
StatePublished - 1985

Keywords

  • prostate regression
  • rat prostate lobes
  • two‐dimensional protein electrophoresis

ASJC Scopus subject areas

  • Oncology
  • Urology

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