@article{26e3452c3c754cee8fd7e4f2c8be1b45,
title = "Proteoform-Selective Imaging of Tissues Using Mass Spectrometry**",
abstract = "Unraveling the complexity of biological systems relies on the development of new approaches for spatially resolved proteoform-specific analysis of the proteome. Herein, we employ nanospray desorption electrospray ionization mass spectrometry imaging (nano-DESI MSI) for the proteoform-selective imaging of biological tissues. Nano-DESI generates multiply charged protein ions, which is advantageous for their structural characterization using tandem mass spectrometry (MS/MS) directly on the tissue. Proof-of-concept experiments demonstrate that nano-DESI MSI combined with on-tissue top-down proteomics is ideally suited for the proteoform-selective imaging of tissue sections. Using rat brain tissue as a model system, we provide the first evidence of differential proteoform expression in different regions of the brain.",
keywords = "Mass Spectrometry Imaging, On-Tissue Top-down Proteomics, Post-Translational Modifications, Proteoforms",
author = "Manxi Yang and Hang Hu and Pei Su and Thomas, {Paul M.} and Camarillo, {Jeannie M.} and Greer, {Joseph B.} and Early, {Bryan P.} and Fellers, {Ryan T.} and Kelleher, {Neil L.} and Julia Laskin",
note = "Funding Information: The authors gratefully acknowledge the financial support from the National Institutes of Health (NIH) Common Fund, through the Office of Strategic Coordination/Office of the NIH Director under awards UG3HL145593 and UH3CA255132 (HuBMAP Program, J.L.), UH3CA246635 (HuBMAP Program, N.L.K.), and P41GM108569 (N.L.K.), and the support from National Science Foundation (NSF) under awards #1916691 (Center for Bioanalytic Metrology, J.L.). MSI data analysis tools used in this study were developed with support from NSF-1808136 and NSF-2108729 (J.L.). We thank Drs. Elizabeth K. Neumann and Jeffrey M. Spraggins (Vanderbilt University) for providing rat brain tissue sections. Funding Information: The authors gratefully acknowledge the financial support from the National Institutes of Health (NIH) Common Fund, through the Office of Strategic Coordination/Office of the NIH Director under awards UG3HL145593 and UH3CA255132 (HuBMAP Program, J.L.), UH3CA246635 (HuBMAP Program, N.L.K.), and P41GM108569 (N.L.K.), and the support from National Science Foundation (NSF) under awards #1916691 (Center for Bioanalytic Metrology, J.L.). MSI data analysis tools used in this study were developed with support from NSF‐1808136 and NSF‐2108729 (J.L.). We thank Drs. Elizabeth K. Neumann and Jeffrey M. Spraggins (Vanderbilt University) for providing rat brain tissue sections. Publisher Copyright: {\textcopyright} 2022 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.",
year = "2022",
month = jul,
day = "18",
doi = "10.1002/anie.202200721",
language = "English (US)",
volume = "61",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "John Wiley and Sons Ltd",
number = "29",
}