Proteomic analysis of an extreme halophilic archaeon Halobacterium species NRC-1

Young Ah Goo*, Eugene C. Yi, W. Andy Tao, Nitin S. Baliga, Min Pan, David R. Goodlett, Lee Hood, Wailap V. Ng

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The proteins expressed by extreme halophilic archaeon Halobacterium species NRC-1 were identified. Soluble and membrane proteins produced by the organism were collected in different fractions according to their solubility, were denatured, reduced, and finally subjected to tryptic digestion. Mass spectra were then acquired by data-dependent ion selection form a full range as well as discrete narrow survey scan m/z ranges to increase the number of identifications. It was found that a total of 442 proteins had one or more peptides with highly distinguishable MS/MS spectra and relative low noise.

Original languageEnglish (US)
Title of host publicationProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages221-222
Number of pages2
StatePublished - Dec 1 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

ASJC Scopus subject areas

  • Spectroscopy

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    Goo, Y. A., Yi, E. C., Tao, W. A., Baliga, N. S., Pan, M., Goodlett, D. R., Hood, L., & Ng, W. V. (2002). Proteomic analysis of an extreme halophilic archaeon Halobacterium species NRC-1. In Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 221-222)