Proteomic Analysis Uncovers Measles Virus Protein C Interaction with p65–iASPP Protein Complex

Alice Meignié, Chantal Combredet, Marc Santolini, István A. Kovács, Thibaut Douché, Quentin Giai Gianetto, Hyeju Eun, Mariette Matondo, Yves Jacob, Regis Grailhe, Frédéric Tangy*, Anastassia V. Komarova

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Viruses manipulate the central machineries of host cells to their advantage. They prevent host cell antiviral responses to create a favorable environment for their survival and propagation. Measles virus (MV) encodes two nonstructural proteins MV-V and MV-C known to counteract the host interferon response and to regulate cell death pathways. Several molecular mechanisms underlining MV-V regulation of innate immunity and cell death pathways have been proposed, whereas MV-C host-interacting proteins are less studied. We suggest that some cellular factors that are controlled by MV-C protein during viral replication could be components of innate immunity and the cell death pathways. To determine which host factors are targeted by MV-C, we captured both direct and indirect host-interacting proteins of MV-C protein. For this, we used a strategy based on recombinant viruses expressing tagged viral proteins followed by affinity purification and a bottom-up mass spectrometry analysis. From the list of host proteins specifically interacting with MV-C protein in different cell lines, we selected the host targets that belong to immunity and cell death pathways for further validation. Direct protein interaction partners of MV-C were determined by applying protein complementation assay and the bioluminescence resonance energy transfer approach. As a result, we found that MV-C protein specifically interacts with p65–iASPP protein complex that controls both cell death and innate immunity pathways and evaluated the significance of these host factors on virus replication.

Original languageEnglish (US)
Article number100049
JournalMolecular and Cellular Proteomics
StatePublished - 2021


  • Measles virus (MV) is a member of the genus Morbillivirus of the family Paramyxoviridae. This enveloped virus with a

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology


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