Proteomics identification of Drosophila small interfering RNA-associated factors

Vincent R. Gerbasi, Daniel E. Golden, Sara B. Hurtado, Erik J. Sontheimer

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The Drosophila melanogaster RNA-induced silencing complex (RISC) forms a large ribonucleoprotein particle on small interfering RNAs (siRNAs) and catalyzes target mRNA cleavage during RNA interference (RNAi). Dicer-2, R2D2, Loquacious, and Argonaute-2 are examples of RISC-associated factors that are involved in RNAi. Holo-RISC is an ∼80 S small interfering ribonucleoprotein, which suggests that there are many additional proteins that participate in the RNAi pathway. In this study, we used siRNA affinity capture combined with mass spectrometry to identify novel components of the Drosophila RNAi machinery. Our study identified both established RISC components and novel siRNA-associated factors, many of which contain domains that are consistent with potential roles in RNAi. Functional analysis of these novel siRNA-associated proteins suggests that these factors may play an important role in RNAi.

Original languageEnglish (US)
Pages (from-to)1866-1872
Number of pages7
JournalMolecular and Cellular Proteomics
Volume9
Issue number9
DOIs
StatePublished - Sep 2010

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology

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