Protocol for tissue clearing and 3D analysis of dopamine neurons in the developing mouse midbrain

Youri Adolfs, Divya D.A. Raj, Sara Brignani, R. Jeroen Pasterkamp*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Advances in tissue clearing enable analysis of complex migratory patterns of developing neurons in whole intact tissue. Here, we implemented a modified version of 3DISCO to study migration of midbrain dopamine (DA) neurons. We provide a detailed protocol starting from whole-brain immunostaining, tissue clearing, and ultramicroscopic imaging to post-acquisition quantification and analysis. This protocol enables precise quantification of DA neuron migration but can also be applied more generally for analyzing neuron migration throughout the nervous system. For complete details on the use and execution of this protocol, please refer to Brignani et al. (2020).

Original languageEnglish (US)
Article number100669
JournalSTAR Protocols
Volume2
Issue number3
DOIs
StatePublished - Sep 17 2021
Externally publishedYes

Keywords

  • Antibody
  • Microscopy
  • Model Organisms
  • Molecular Biology
  • Neuroscience

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)

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