Protocol to map multi-transmitter neurons in the mouse brain using intersectional strategy

Jian Xu; Anis Contractor; Yongling Zhu

doi:10.1016/j.xpro.2022.101907

Protocol to map multi-transmitter neurons in the mouse brain using intersectional strategy

Jian Xu, Anis Contractor, Yongling Zhu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Although it is now known that certain neurons can produce, store, and release multiple neurotransmitters, their locations, abundance, and functions remain elusive. We developed intersectional genetic strategies to identify multi-transmitter neurons based on the expression of neurotransmitter-specific genes. Here we present our procedures for whole-brain mapping of GABA/glutamate co-releasing neurons. We also detail our technique for labeling GABA/glutamate neurons in specific brain regions with adeno-associated virus (AAV). Our protocol can be readily extended to other types of multi-transmitter neurons. For complete details on the use and execution of this protocol, please refer to Xu et al. (2022).¹

Original language	English (US)
Article number	101907
Journal	STAR Protocols
Volume	3
Issue number	4
DOIs	https://doi.org/10.1016/j.xpro.2022.101907
State	Published - Dec 16 2022

Funding

This work was supported by NIH grants R01 EY030169 (Y.Z.), R01 EY018204 (Y.Z.), Whitehall Foundation Grant (Y.Z., 2017-05-20 ), R01 MH099114 (A.C.), R01 EY032506 (Y.Z. and A.C.), and Research to Prevent Blindness .

Keywords

Genetics
Neuroscience

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology
General Neuroscience

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AB - Although it is now known that certain neurons can produce, store, and release multiple neurotransmitters, their locations, abundance, and functions remain elusive. We developed intersectional genetic strategies to identify multi-transmitter neurons based on the expression of neurotransmitter-specific genes. Here we present our procedures for whole-brain mapping of GABA/glutamate co-releasing neurons. We also detail our technique for labeling GABA/glutamate neurons in specific brain regions with adeno-associated virus (AAV). Our protocol can be readily extended to other types of multi-transmitter neurons. For complete details on the use and execution of this protocol, please refer to Xu et al. (2022).1

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Protocol to map multi-transmitter neurons in the mouse brain using intersectional strategy

Abstract

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Keywords

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