Protocols for growth and development of Dictyostelium discoideum

Petra Fey, Anthony S. Kowal, Pascale Gaudet, Karen E. Pilcher, Rex L. Chisholm*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

168 Scopus citations


Dictyostelium discoideum, a unicellular organism capable of developing into a multicellular structure, is a powerful model system to study a variety of biological processes. Because it is inexpensive and relatively easy to grow, Dictyostelium is also frequently used in teaching laboratories. Here we describe conditions for successfully growing and developing Dictyostelium cells and methods for long-term storage of Dictyostelium amoebae and spores.

Original languageEnglish (US)
Pages (from-to)1307-1316
Number of pages10
JournalNature Protocols
Issue number6
StatePublished - Jun 2007

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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