Protocols for growth and development of Dictyostelium discoideum

Petra Fey, Anthony S. Kowal, Pascale Gaudet, Karen E. Pilcher, Rex L. Chisholm*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

130 Scopus citations

Abstract

Dictyostelium discoideum, a unicellular organism capable of developing into a multicellular structure, is a powerful model system to study a variety of biological processes. Because it is inexpensive and relatively easy to grow, Dictyostelium is also frequently used in teaching laboratories. Here we describe conditions for successfully growing and developing Dictyostelium cells and methods for long-term storage of Dictyostelium amoebae and spores.

Original languageEnglish (US)
Pages (from-to)1307-1316
Number of pages10
JournalNature Protocols
Volume2
Issue number6
DOIs
StatePublished - Jun 1 2007

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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