Protocols for Structural and Functional Analysis of Particulate Methane Monooxygenase from Methylocystis Species Strain Rockwell (ATCC 49242)

Sarah Sirajuddin, Amy C Rosenzweig

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Particulate methane monooxygenase (pMMO) is a membrane-bound metalloenzyme that oxidizes methane to methanol in methanotrophic bacteria, organisms that consume methane as their sole carbon and energy source. In order to understand the biochemistry of methane oxidation, pMMO must be isolated from the native organism. This chapter describes protocols for growth, isolation, and characterization of pMMO from Methylocystis species strain Rockwell.
Original languageEnglish (US)
Title of host publicationHydrocarbon and Lipid Microbiology Protocols
Subtitle of host publicationBiochemical Methods
EditorsTerry J McGenity, Kenneth N Timmis, Balbina Nogales
PublisherSpringer Berlin Heidelberg
Pages149-160
Number of pages12
ISBN (Electronic)978-3-662-49137-9
ISBN (Print)978-3-662-49135-5
StatePublished - 2014

Publication series

NameSpringer Protocols Handbooks
ISSN (Print)1949-2448

Fingerprint Dive into the research topics of 'Protocols for Structural and Functional Analysis of Particulate Methane Monooxygenase from Methylocystis Species Strain Rockwell (ATCC 49242)'. Together they form a unique fingerprint.

  • Cite this

    Sirajuddin, S., & Rosenzweig, A. C. (2014). Protocols for Structural and Functional Analysis of Particulate Methane Monooxygenase from Methylocystis Species Strain Rockwell (ATCC 49242). In T. J. McGenity, K. N. Timmis, & B. Nogales (Eds.), Hydrocarbon and Lipid Microbiology Protocols: Biochemical Methods (pp. 149-160). (Springer Protocols Handbooks). Springer Berlin Heidelberg.