Protocols for Structural and Functional Analysis of Particulate Methane Monooxygenase from Methylocystis Species Strain Rockwell (ATCC 49242)

Sarah Sirajuddin; Amy C Rosenzweig

Protocols for Structural and Functional Analysis of Particulate Methane Monooxygenase from Methylocystis Species Strain Rockwell (ATCC 49242)

Molecular Biosciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Particulate methane monooxygenase (pMMO) is a membrane-bound metalloenzyme that oxidizes methane to methanol in methanotrophic bacteria, organisms that consume methane as their sole carbon and energy source. In order to understand the biochemistry of methane oxidation, pMMO must be isolated from the native organism. This chapter describes protocols for growth, isolation, and characterization of pMMO from Methylocystis species strain Rockwell.

Original language	English (US)
Title of host publication	Hydrocarbon and Lipid Microbiology Protocols
Subtitle of host publication	Biochemical Methods
Editors	Terry J McGenity, Kenneth N Timmis, Balbina Nogales
Publisher	Springer Berlin Heidelberg
Pages	149-160
Number of pages	12
ISBN (Electronic)	978-3-662-49137-9
ISBN (Print)	978-3-662-49135-5
State	Published - 2014

Publication series

Name	Springer Protocols Handbooks
ISSN (Print)	1949-2448

Cite this

Sirajuddin, S., & Rosenzweig, A. C. (2014). Protocols for Structural and Functional Analysis of Particulate Methane Monooxygenase from Methylocystis Species Strain Rockwell (ATCC 49242). In T. J. McGenity, K. N. Timmis, & B. Nogales (Eds.), Hydrocarbon and Lipid Microbiology Protocols: Biochemical Methods (pp. 149-160). (Springer Protocols Handbooks). Springer Berlin Heidelberg.

Sirajuddin, Sarah ; Rosenzweig, Amy C. / Protocols for Structural and Functional Analysis of Particulate Methane Monooxygenase from Methylocystis Species Strain Rockwell (ATCC 49242). Hydrocarbon and Lipid Microbiology Protocols: Biochemical Methods. editor / Terry J McGenity ; Kenneth N Timmis ; Balbina Nogales. Springer Berlin Heidelberg, 2014. pp. 149-160 (Springer Protocols Handbooks).

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title = "Protocols for Structural and Functional Analysis of Particulate Methane Monooxygenase from Methylocystis Species Strain Rockwell (ATCC 49242)",

abstract = "Particulate methane monooxygenase (pMMO) is a membrane-bound metalloenzyme that oxidizes methane to methanol in methanotrophic bacteria, organisms that consume methane as their sole carbon and energy source. In order to understand the biochemistry of methane oxidation, pMMO must be isolated from the native organism. This chapter describes protocols for growth, isolation, and characterization of pMMO from Methylocystis species strain Rockwell.",

author = "Sarah Sirajuddin and Rosenzweig, {Amy C}",

year = "2014",

language = "English (US)",

isbn = "978-3-662-49135-5",

series = "Springer Protocols Handbooks",

publisher = "Springer Berlin Heidelberg",

pages = "149--160",

editor = "McGenity, {Terry J} and Timmis, {Kenneth N} and Balbina Nogales",

booktitle = "Hydrocarbon and Lipid Microbiology Protocols",

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Protocols for Structural and Functional Analysis of Particulate Methane Monooxygenase from Methylocystis Species Strain Rockwell (ATCC 49242). / Sirajuddin, Sarah; Rosenzweig, Amy C.
Hydrocarbon and Lipid Microbiology Protocols: Biochemical Methods. ed. / Terry J McGenity; Kenneth N Timmis; Balbina Nogales. Springer Berlin Heidelberg, 2014. p. 149-160 (Springer Protocols Handbooks).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

TY - CHAP

T1 - Protocols for Structural and Functional Analysis of Particulate Methane Monooxygenase from Methylocystis Species Strain Rockwell (ATCC 49242)

AU - Sirajuddin, Sarah

AU - Rosenzweig, Amy C

PY - 2014

Y1 - 2014

N2 - Particulate methane monooxygenase (pMMO) is a membrane-bound metalloenzyme that oxidizes methane to methanol in methanotrophic bacteria, organisms that consume methane as their sole carbon and energy source. In order to understand the biochemistry of methane oxidation, pMMO must be isolated from the native organism. This chapter describes protocols for growth, isolation, and characterization of pMMO from Methylocystis species strain Rockwell.

AB - Particulate methane monooxygenase (pMMO) is a membrane-bound metalloenzyme that oxidizes methane to methanol in methanotrophic bacteria, organisms that consume methane as their sole carbon and energy source. In order to understand the biochemistry of methane oxidation, pMMO must be isolated from the native organism. This chapter describes protocols for growth, isolation, and characterization of pMMO from Methylocystis species strain Rockwell.

M3 - Chapter

SN - 978-3-662-49135-5

T3 - Springer Protocols Handbooks

SP - 149

EP - 160

BT - Hydrocarbon and Lipid Microbiology Protocols

A2 - McGenity, Terry J

A2 - Timmis, Kenneth N

A2 - Nogales, Balbina

PB - Springer Berlin Heidelberg

ER -

Protocols for Structural and Functional Analysis of Particulate Methane Monooxygenase from Methylocystis Species Strain Rockwell (ATCC 49242)

Abstract

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